云南地区武定鸡白血病病毒受体TVB基因型的检测

发布时间：2018-01-27 00:23

  本文关键词： 武定鸡 禽白血病 病毒受体 TVB　出处：《中国家禽》2017年09期 　论文类型：期刊论文

【摘要】：通过了解武定鸡群白血病病毒受体TVB基因型分布情况,为下一步研究ALV-B、D、E亚群禽白血病抗病育种奠定基础。试验以云南地区三个不同养鸡场的168日龄武定鸡为研究对象,随机分成三个平行组,每组200只。采用PCR-RFLP的方法对TVB受体基因进行检测。结果:在武定鸡群中TVB*S1/S1基因型频率较高,频率为0.28~0.355;TVB*RR基因型频率较低,频率为0.03~0.065;而其他基因型频率中等,频率为0.120~0.205。在各组中TVB*S1等位基因频率最高,为0.563;最低为TVB*R等位基因,频率为0.115;TVB*S3频率介于TVB*S1和TVB*R两者之间。在这3组内TVB*S1/R、TVB*S3/R和TVB*S3/S3基因型之间频率无显著性差异(P0.05),TVB*S1/S3、TVB*S1/S1和TVB*R/R基因型频率低于前面三种基因型,高于TVB*R/R基因型。TVB*S1/S1基因型频率在组内最高,显著高于其他五种基因型(P0.05),TVB*S1/S3基因型次之。结果表明:在武定鸡群中易感B、D、E亚型禽白血病病毒的基因频率较高,而抗B、D、E亚群禽白血病毒的基因频率在中等水平,为后续抗B、D、E亚群禽白血病病毒选育工作提供遗传背景资料。
[Abstract]:To investigate the distribution of TVB genotypes of Leukemia virus receptor (TVB) in Wuding chickens, and to study ALV-BUD for the next step. The 168-day-old Wuding chickens from three different chicken farms in Yunnan Province were randomly divided into three parallel groups. The TVB receptor gene was detected by PCR-RFLP. Results: the frequency of TVB*S1/S1 genotype was higher in Wuding chickens. The frequency is 0.28 ~ 0.355; The frequency of TVB*RR genotype was low, 0.03 ~ 0.065; The frequency of other genotypes was moderate (0.120) 0.205. The frequency of TVB*S1 allele was the highest (0.563) in each group. The lowest allele was TVB*R, the frequency was 0.115; The frequency of TVB*S3 was between TVB*S1 and TVB*R. TVB*S1/R was found in these three groups. There was no significant difference in frequency between TVB*S3/R and TVB*S3/S3 genotypes. The frequency of TVB*S1/S1 and TVB*R/R genotypes was lower than that of the former three genotypes, and higher than that of TVB*R/R genotypes. It was significantly higher than that of the other five genotypes (P0.05TV-S1 / S3). The results showed that the gene frequency of the susceptible avian leukemia virus was higher in Wuding chickens than that in the other five genotypes. However, the gene frequency of avian leukemia virus resistant to subgroup BGDU E was at a moderate level, which provided genetic background information for the subsequent selection of avian leukemia virus resistant to subgroup BGDU E.
【作者单位】： 云南农业大学动物科学技术学院;云南农大科技生物公司剑川分公司;曲靖市宣威龙潭镇农业综合服务中心;云南农村干部学院;
【基金】：高原优质地方武定鸡推广与养殖技术服务资助(43120331X)
【分类号】：S858.31
【正文快照】： 禽白血病(Avian leukosis,AL)是由禽α型反转TVB*S1的易感性最强。而且,TVB*S1对TVB*S3录病毒群的病毒引起的禽类多种肿瘤性疾病的统和TVB*R是完全显性,TVB*S3对TVB*R是完全称。目前已经鉴定出四种鸡白血病病毒细胞受体显性[6]。因此在鸡群中检测TVB的基因型,对于鸡(Tumor vir，

本文编号：1467021