大叶落地生根细胞周期蛋白依赖激酶KdCDK基因克隆及表达分析

发布时间：2018-01-27 00:15

本文关键词： 大叶落地生根细胞周期蛋白依赖激酶基因表达　出处：《华北农学报》2017年03期 　论文类型：期刊论文

【摘要】：为了更好理解大叶落地生根中细胞周期调控的分子机制,利用RACE-PCR技术从大叶落地生根中克隆了1个新的基因KdCDK。该基因编码295个氨基酸残基,分子量为34.12kDa,等电点为6.15,其开放阅读框长度为888bp。其蛋白与笋瓜CDK蛋白的同源性最高,属于CDKA类蛋白家族。实时荧光定量PCR分析表明,该蛋白基因在大叶落地生根的根中表达量最高,且受渗透胁迫(甘露醇)的诱导上调表达。从大叶落地生根中克隆出KdCDK基因,为将来该基因的功能研究打下了基础。
[Abstract]:In order to better understand the molecular mechanism of cell cycle regulation in rooting. A new gene, KdCDKK, was cloned from rooting in large leaves by RACE-PCR technique. The gene encodes 295 amino acid residues with a molecular weight of 34.12 kDa. The isoelectric point was 6.15 and the length of the open reading frame was 888bp.The protein had the highest homology with the CDK protein of bamboo shoots melon and belonged to the CDKA protein family. The real-time fluorescence quantitative PCR analysis showed that the protein homology was the highest. The expression of the protein gene was the highest in the rooting roots and up-regulated by osmotic stress (mannitol). The KdCDK gene was cloned from the rooting of Lepidopsis macrophylla. It will lay a foundation for the study of the function of this gene in the future.
【作者单位】：深圳市日f园林绿化有限公司;北京林业大学林学院草坪研究所;暨南大学深圳旅游学院;华南农业大学林学与风景园林学院广东省草业工程技术研究中心;
【基金】：深圳市科技计划项目(CXZZ20140418110342522) 北京林业大学与厦门日懋城建园林建设股份有限公司产学研合作项目
【分类号】：S682.33
【正文快照】： 3.暨南大学深圳旅游学院,广东深圳518053;4.华南农业大学林学与风景园林学院,广东省草业工程技术研究中心,广东广州510642)细胞周期是受到严格调控的过程,通过一系列不连续的关键点来确保DNA正确复制和细胞的成功分裂。真核生物中的细胞周期由丝氨酸/苏氨酸蛋白激酶家族调控,

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