辽宁地区水稻资源抗稻瘟病基因的检测分析

发布时间：2018-01-26 23:43

本文关键词： 水稻品种稻瘟病表型鉴定基因型鉴定基因聚合　出处：《植物遗传资源学报》2017年02期 　论文类型：期刊论文

【摘要】：为了明确辽宁地区水稻资源中抗稻瘟病基因的分布情况及抗病效应,选取辽宁地区水稻资源176份,鉴定了抗稻瘟病基因pi21、Pi36、Pi37、Pita、Pid2、Pid3、Pi5及Pib在这些材料中的分布情况,并接种鉴定了这些材料对稻瘟病的抗性。结果表明:176份供试材料中,83份对稻瘟病表现抗病,栽培稻、杂草稻及农家种中抗病品种所占的比率分别为41.48%、1.14%及4.54%。抗稻瘟病基因pi21、Pi36和Pi37在所有参试材料中均未检测到,且分别有74份、49份、47份、52份及89份材料携带Pita、Pid2、Pid3、Pi5及Pib的抗病等位基因。抗病基因绝大部分分布在栽培种中,农家种和杂草稻中分布较少。不含有抗稻瘟病基因和只携带单个抗病基因的材料对稻瘟病的抗性均较差,而抗病基因聚合可不同程度提高材料的抗性。经检测,不含有本试验鉴定的pi21等8个已克隆抗病基因的材料共32份,其中表现抗病的占21.87%;只携带1个抗稻瘟病基因的材料为52份,表现抗病的占17.31%;携带2个抗稻瘟病基因的材料为39份,表现抗病的占69.23%,其中以携带Pita+Pi5的材料最多(14份),且均表现抗病;携带3个抗稻瘟病基因的材料为31份,表现抗病的占77.42%,以携带Pita+Pid3+Pi5的材料抗性最强;携带4个抗稻瘟病基因的水稻材料22份,表现抗病的占72.73%,携带5个抗病基因的水稻材料未检测到。
[Abstract]:In order to determine the distribution of rice blast resistance genes and the resistance effect of rice in Liaoning province, 176 rice resources in Liaoning province were selected to identify the rice blast resistance gene pi21, Pi36, Pi37. The distribution of Pid3 Pi5 and Pib in these materials was studied, and the resistance of these materials to blast was identified by inoculation. The results showed that 1: 176 tested materials were resistant to rice blast. The percentage of resistant varieties in cultivated rice, weedy rice and farm species was 41.48% and 4.54%, respectively. The blast resistance gene pi21 was found in 83 rice varieties. Pi36 and Pi37 were not detected in all the tested materials, and there were 74 samples, 49 samples, 47 samples, 52 samples, and 89 samples, which carried Pita Pid2 Pid3, respectively. The resistance alleles of Pi5 and Pib were mostly distributed in cultivated species. The resistance of rice blast resistant gene and single resistance gene were poor, but resistance gene polymerization could improve resistance to rice blast in different degree. There were 32 clones of 8 cloned resistant genes, such as pi21, which were not identified in this experiment, of which 21.87 were resistant to disease. Only one blast resistance gene was carried in 52 materials, and 17.31% of them showed resistance to rice blast. There were 39 rice blast resistant genes, and the resistant materials were 69.23. The most of them were Pita Pi5, and all of them were resistant to rice blast. Among 31 rice blast resistant genes, the resistant materials were 77.422.The materials carrying Pita Pid3 Pi5 were the most resistant. 22 rice lines with 4 rice blast resistance genes were found to be resistant to rice blast, and 72.73 rice lines with 5 resistance genes were not detected.
【作者单位】：沈阳农业大学水稻研究所;辽宁省农业科学院创新中心;辽宁省水稻研究所;
【基金】：国家自然科学基金项目(31571993) 公益性行业(农业)科研专项课题(201203026-3) 国家“863”计划项目(2014AA10A603)
【分类号】：S435.111.41
【正文快照】： URL:http://www.cnki.net/kcms/detail/11.4996.S.20170217.1107.006.html水稻是世界上最重要的粮食作物之一,世界上超过一半的人口以稻米为食。水稻是中国粮食安全和农业可持续发展的重要战略资源[1-3]。由稻瘟病菌(无性态:Pyricularia grisea Sacc.)引起的稻瘟病,是水稻生产

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