中华蜜蜂幼虫肠道的高表达基因分析

发布时间：2018-01-28 05:19

本文关键词： RNA-seq 中华蜜蜂幼虫肠道高表达基因　出处：《浙江农业学报》2017年09期 　论文类型：期刊论文

【摘要】：中华蜜蜂(中蜂)是中国特有的本土蜜蜂资源,也是养蜂生产中的常用蜂种之一,具有重要的生态和经济价值。本研究利用RNA-seq技术对中蜂5和6日龄幼虫肠道进行深度测序,得到的有效读段(clean reads)数分别为29290630与26636038,Q20分别为98.45%和98.38%。GO富集分析结果显示,Ac5中的高表达基因(HEGs)富集在44个GO term,其中基因富集数最多的为代谢进程(753 unigenes),其次是催化活性(679 unigenes)与结合(657 unigenes);Ac6中的HEGs富集在44个GO term,其中基因富集数最多的为代谢进程(792 unigenes),其次是结合(760 unigenes)和催化活性(744 unigenes)。KEGG代谢通路(pathway)富集分析结果显示,Ac5的HEGs富集在129个pathway,其中基因富集数最多的是核糖体(103 unigenes)、内质网蛋白质加工(68 unigenes)和碳代谢(67 unigenes);Ac6的HEGs富集在129个代谢通路,其中基因富集数最多的是核糖体(103 unigenes)、氧化磷酸化(88 unigenes)及内质网蛋白质加工(68 unigenes)。进一步对HEGs进行Venn分析,结果显示,二者共有的HEGs为2 015个,特有的HEGs分别为219和491个。研究结果不仅可提供中蜂幼虫肠道发育过程中的基因表达谱数据,也为分子水平上深入研究中蜂幼虫肠道发育提供重要信息。
[Abstract]:Chinese bee (Chinese bee) is a unique native bee resource in China, and it is also one of the commonly used bee species in beekeeping production. It has important ecological and economic value. In this study, RNA-seq technique was used to carry out deep sequencing of the intestinal tract of the 5th and 6th day-old larvae. The number of valid read segments clean read is 29290630 and 26636038, respectively. Q20 was 98.45% and 98.38%, respectively. The results of enrichment analysis showed that the high expression gene (hEGs) in Ac5 was enriched in 44 go term. The most abundant genes were 753 unigenesus, followed by catalytic activity of 679 unigeneses) and binding of 657 unigeneses. The HEGs in Ac6 was enriched in 44 go terms.Among them, the most abundant gene was metabolic process (792 unigenes). The second one was combined with 760unigenes) and the catalytic activity was 744 unigenes).KEGG pathway. The HEGs of Ac5 was enriched at 129 pathways, and the most abundant gene was ribosome 103unigenes. Endoplasmic reticulum protein processing (68 unigenes) and carbon metabolism 67 unigenesus; The HEGs of Ac6 was enriched in 129 metabolic pathways, and the most abundant gene was ribosome 103unigenes. Oxidative phosphorylation 88 unigenes) and endoplasmic reticulum protein processing 68 unigenes.Furthermore, Venn analysis of HEGs showed that. There were 2 015 HEGs and 219 and 491 unique HEGs, respectively. The results could not only provide the gene expression profile data during the intestinal development of the larvae. It also provides important information for the further study of the intestinal development of wasp larvae at the molecular level.
【作者单位】：福建农林大学蜂学学院;
【基金】：现代农业产业技术体系建设专项基金(CARS-45-KXJ7) 福建农林大学科技发展基金(KF2015123) 福建省大学生创新创业项目(201610389053)
【分类号】：S891
【正文快照】： 东方蜜蜂(Apis cerana)主要集中分布于亚洲的中国、日本、泰国、菲律宾和伊朗等国家,以及俄罗斯远东地区[1]。中华蜜蜂(Apis cerana cera-na,简称中蜂)特指分布在我国的东方蜜蜂,其距今已有3 000多年的驯养历史[2]。中蜂是我国重要的本土蜜蜂资源,也是养蜂生产中的常用蜂种之

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