烟碱型乙酰胆碱受体α7在Fmr1基因敲除小鼠中的表达及意义

发布时间：2018-01-28 09:34

本文关键词： Fmr基因敲除跳台实验学习记忆 α烟碱型乙酰胆碱受体　出处：《解剖学研究》2016年06期 　论文类型：期刊论文

【摘要】：目的观察烟碱型乙酰胆碱受体α7在Fmr1基因敲除小鼠前额皮层及海马的表达,探讨其在Fmr1基因敲除小鼠学习记忆中的作用。方法将4周龄FVB小鼠分为WT组和KO组,用跳台实验分别检测KO及WT小鼠学习记忆的能力,利用Western blotting和免疫组化方法检测KO及WT小鼠前额皮层及海马α7 nAChR的表达。结果跳台实验结果显示,与WT空白组比较,KO空白组第一天及第二天的潜伏期均显著缩短,错误次数明显增多,差异均具有统计学意义(P0.05);免疫组化结果显示,与WT空白组相比,KO空白组前额皮层和海马区α7 nAChR免疫阳性细胞数均显著减少(P0.05);Western Blotting结果显示,KO空白组前额皮层及海马区α7 nAChR表达量均较WT空白组减少,海马区更为显著,差异均具有统计学意义(P0.05)。结论 Fmr1基因敲除小鼠前额皮层、海马区的α7 nAChR表达量较野生型小鼠减少,提示α7 nAChR参与了Fmr1基因敲除小鼠学习记忆障碍的发病机制。
[Abstract]:Objective to observe the expression of nicotinic acetylcholine receptor 伪 7 in prefrontal cortex and hippocampus of Fmr1 knockout mice. Methods the 4-week-old FVB mice were divided into WT group and KO group. The ability of learning and memory of KO and WT mice were tested by bench test. The expression of 伪 7 nAChR in prefrontal cortex and hippocampus of KO and WT mice was detected by Western blotting and immunohistochemistry. Compared with WT group, the incubation period of the first day and the second day of KO blank group were significantly shortened, the number of errors increased obviously, the difference was statistically significant (P 0.05). The results of immunohistochemistry showed that the number of 伪 7 nAChR immunoreactive cells in prefrontal cortex and hippocampal area in KO blank group was significantly lower than that in WT blank group (P 0.05). The results of Western Blotting showed that the expression of 伪 7 nAChR in prefrontal cortex and hippocampal area in the control group was lower than that in the WT control group, and the expression of 伪 7 nAChR in the hippocampus was more significant than that in the control group. Conclusion the expression of 伪 7 nAChR in prefrontal cortex and hippocampus of Fmr1 knockout mice is lower than that of wild-type mice. These results suggest that 伪 7 nAChR is involved in the pathogenesis of learning and memory impairment in Fmr1 knockout mice.
【作者单位】：广州医科大学附属第二医院神经内科;广州医科大学附属第二医院神经科学研究所;
【基金】：广东省省级科技计划项目(2014A020212317)1
【分类号】：R596.1
【正文快照】： 脆性X综合征(fragile X syndrome,FXS)是最常见的X连锁单基因遗传性智力低下疾病之一,其X连锁的脆性X智力低下1号基因(fragile X mental retardation gene 1,Fmr1)5′端非翻译区(CGG)n三核苷酸重复序列异常扩增及其相邻部位Cp G岛的异常甲基化,导致其编码产物脆性X智力低下蛋

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