上饶市乙型流感病毒耐药基因分析

发布时间：2018-01-28 11:47

本文关键词： 乙型流感病毒耐药神经氨酸酶基因　出处：《中国感染与化疗杂志》2017年05期 　论文类型：期刊论文

【摘要】：目的分析上饶市乙型流感病毒耐药基因,为临床用药和疾病控制提供依据。方法从哨点医院采集疑似流感患者咽拭子样本,接种犬肾传代细胞(Madin-Darby Canine Kidney,MDCK)及进行分离培养。选择分离到的乙型(即B型)流感病毒株进行核酸提取后进行反转录-聚合酶链反应(RT-PCR),扩增神经氨酸酶(neuraminidase,NA)基因并进行核苷酸测序,采用DNA Star6.0、Mage5.0生物软件对测序结果进行分析处理,推导出氨基酸序列,进行基因特性分析。结果7株乙型流感病毒NA基因同源性高达99.5%以上,NA蛋白催化活性位点和辅助位点均无氨基酸替换。结论 7株毒株均对流感病毒NA抑制剂药物敏感,但仍应加强对流感病毒的耐药性监测。
[Abstract]:Objective to analyze the drug resistance genes of influenza B virus in Shangrao city, and to provide evidence for clinical use and disease control. Methods throat swabs of suspected influenza patients were collected from sentinel hospitals. Canine kidney passage cells were inoculated with Madin-Darby Canine Kidney. The isolated influenza B (B) strain was selected for nucleic acid extraction and reverse transcription-polymerase chain reaction (RT-PCR). The neuraminidase (neuraminidase) gene was amplified and sequenced by DNA Star6.0. Mage5.0 software analyzed the sequencing results, deduced the amino acid sequence and analyzed the gene characteristics. The results showed that the na gene homology of 7 strains of influenza B virus was more than 99.5%. Conclusion all the 7 strains are sensitive to na inhibitor of influenza virus, but should strengthen the surveillance of resistance to influenza virus.
【作者单位】：上饶市疾病预防控制中心检验科;江西医学高等专科学校药学系;江西省疾病预防控制中心检验科;
【基金】：2014年江西省医学学科省市共建计划项目(201412)
【分类号】：R440;R511.7
【正文快照】： 作者单位:1.上饶市疾病预防控制中心检验科,江西上饶334000;2.江西医学高等专科学校药学系;3.江西省疾病预防控制中心检验科。流感病毒分为甲型、乙型和丙型3类,以甲型和乙型流感病毒最为常见。其中乙型即B型流感病毒是感染人类的主要病原体之一,虽然对人类致病性较低,呈暴发

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