儿童呼吸道分离流感嗜血杆菌的耐药性和基因分型

发布时间：2018-01-28 19:20

  本文关键词： 流感嗜血杆菌 耐药性 ftsI基因　出处：《中国感染与化疗杂志》2017年05期 　论文类型：期刊论文

【摘要】：目的研究儿童呼吸道分离流感嗜血杆菌的耐药性和该菌ftsI基因分型与耐药表型的关系。方法收集2016年第一季度住院患儿鼻咽吸出物中分离到的流感嗜血杆菌141株;用纸片扩散法检测细菌对抗菌药物的耐药性;用Nitrocefin纸片法检测细菌的β内酰胺酶;用PCR技术对分离菌株进行ftsI基因检测;比较不同基因型菌株对抗菌药物的耐药情况。结果141株流感嗜血杆菌β内酰胺酶检出率为40.4%(57/141)、氨苄西林耐药率为53.2%(75/141)。检出ftsI基因突变率为72.3%(102/141),以Ⅲ型为主(72/102,70.6%)。β内酰胺酶基因阴性氨苄西林耐药型菌株(g BLNAR)对氨苄西林和头孢呋辛的耐药率高于β内酰胺酶基因阴性氨苄西林敏感型菌株(g BLNAS)(P0.05)。结论儿童呼吸道分离流感嗜血杆菌ftsI基因突变率高,以Ⅲ型为主。ftsI基因突变增加了流感嗜血杆菌对氨苄西林和头孢呋辛的耐药性。
[Abstract]:Objective to study the drug resistance of Haemophilus influenzae isolated from respiratory tract in children and the relationship between ftsI genotyping and drug resistance phenotype of Haemophilus influenzae in children. Methods Influenza isolated from nasopharyngeal aspirates of hospitalized children in in the first quarter of 2016 was collected. 141 strains of Haemophilus; The drug resistance of bacteria to antimicrobial agents was detected by disk diffusion method. The 尾 -lactamases of bacteria were detected by Nitrocefin disk method. PCR technique was used to detect the ftsI gene of isolated strains. Results the detection rate of 尾 -lactamases in 141 strains of Haemophilus influenzae was 40.4% or 57 / 141). The resistant rate of ampicillin was 53.2% and 75 / 141% respectively. The mutation rate of ftsI gene was 72.3% and 102% / 141% respectively. The resistant rate of ampicillin and cefuroxime resistant strains with 尾 lactamase gene negative ampicillin was higher than that of 尾 lactamase gene negative ampicillin sensitive strain (. Conclusion the mutation rate of ftsI gene of Haemophilus influenzae isolated from children's respiratory tract is high. The mutation of type 鈪，

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