牦牛TMEM-18基因多态性及其与生产性能关联分析

发布时间：2018-01-28 19:43

  本文关键词： 牦牛 TMEM-基因 SNP HRM 生产性状　出处：《生物技术通报》2017年02期 　论文类型：期刊论文

【摘要】：跨膜蛋白18基因(TMEM-18)是一个末端为低聚嘧啶的基因,与动物生长发育密切相关。为了研究牦牛TMEM-18基因多态性与生产性能的关系。以192头天祝雌性牦牛血样NDA构建混合池,扩增TMEM-18基因内含子1和外显子5的序列。运用BLAST和Chromas软件分析突变位点,运用高分辨率熔解曲线分析技术(HRM)统计基因型分型,采用SHEsis软件进行基因型频率和等位基因频率计算,同时对多态位点进行配对连锁不平衡和单倍型分析,运用SPSS21.0分析基因多态位点与生产性能关联。结果表明,牦牛TMEM-18基因内含子1处存在2个多态位点,分别是861(A/G)和1267(C/T),外显子5处存在1个多态位点为4 447(C/T)。关联分析表明,牦牛TMEM-18基因861(A/G)位点的不同基因型与牦牛的体高、体重、胴体重和屠宰率差异性显著(P0.05);1267(C/T)位点的不同基因型与牦牛的体高、体重和屠宰率差异性显著(P0.05),而牦牛TMEM-18基因4447(C/T)位点的不同基因型与牦牛的体斜长、管围、胸围、体重、胴体重、净肉重、净肉率和屠宰率均存在显著性差异(P0.05)。通过单倍型分析发现群体中存在3种单倍型组合,即ACC单倍型、GTC单倍型和GTT单倍型,其中ACC单倍型组合个体数目明显多于其他单倍型组合,为优势单倍型。本实验揭示TMEM-18基因可作为牦牛生产性能开发的候选分子标记,为牦牛遗传资源的利用、开发与新品种的选育提供依据。
[Abstract]:Transmembrane protein 18 gene (TMEM-18) is a gene with terminal oligopyrimidine. In order to study the relationship between TMEM-18 gene polymorphism and production performance, the mixed pool was constructed from 192 female yak blood samples with NDA. The sequences of intron 1 and exon 5 of TMEM-18 gene were amplified and the mutation sites were analyzed by BLAST and Chromas software. High resolution melting curve analysis technique was used to calculate genotypes, and SHEsis software was used to calculate the frequency of genotypes and alleles. At the same time, the linkage disequilibrium and haplotype of the polymorphic loci were analyzed, and the correlation between the polymorphic loci and the production performance was analyzed by SPSS21.0. There are two polymorphic loci at intron 1 of TMEM-18 gene in yak, which are 861 A / G) and 1267 C / T respectively. One polymorphic locus was found at exon 5. The correlation analysis showed that the different genotypes of the 861 TMEM-18 gene and the body height and body weight of the yak. The difference of carcass weight and slaughter rate was significant (P0.05). Different genotypes of 1267 C / T locus were significantly different from yak body height, body weight and slaughter rate (P0.05). However, different genotypes of TMEM-18 gene 4447 C / T locus were associated with the body length, tube circumference, chest circumference, body weight, carcass weight and net meat weight of yak. There were significant differences in net meat rate and slaughter rate. Haplotype analysis showed that there were three haplotypes in the population, that is, ACC haplotype GTC haplotype and GTT haplotype. The number of individuals of ACC haplotype combinations was significantly more than that of other haplotypes, which was the dominant haplotype. This study revealed that TMEM-18 gene can be used as candidate molecular markers for the development of yak production performance. To provide the basis for utilization, development and breeding of new varieties of yak genetic resources.
【作者单位】： 甘肃农业大学动物医学院;甘肃农业大学生命科学技术学院;甘肃农业大学动物科技学院;
【基金】：“十二五”农村领域国家科技计划课题(2013AA102503-3)
【分类号】：S823.85
【正文快照】： 兰州730070)牦牛(Bos grunniens)是长期放牧于海拔高达3 000 m以上的高寒地区特殊牛种,属于地方类半野生型的种群,是经过长期的自然选择和人工选育而形成的高原特有畜种资源。牦牛既是我国特有的畜种资源,也是我国稀有而珍贵的遗传资源[1]。牦牛生长发育缓慢、群体规模小、生，

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