c-myc蛋白表达水平与c-myc基因易位相关性分析及其临床应用

发布时间：2018-01-29 00:26

  本文关键词： 淋巴瘤 弥漫大B细胞淋巴瘤 c-myc 免疫组织化学 荧光原位杂交　出处：《临床与实验病理学杂志》2016年10期 　论文类型：期刊论文

【摘要】：目的探讨c-myc蛋白表达与c-myc基因易位的相关性,旨在寻找适用于临床c-myc蛋白的界定值,以有效判断c-myc基因易位状态。方法采用免疫组化法与荧光原位杂交(fluorescence in situ hybridization,FISH)法检测293例弥漫大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)中c-myc蛋白表达水平与c-myc基因易位状态,分析两者的相关性。结果 293例DLBCL中,30例(10.2%)发生c-myc基因易位,其中29例c-myc蛋白阳性细胞百分率40%,1例c-myc蛋白阳性细胞百分率为40%,而c-myc蛋白阳性细胞百分率40%者均未发生c-myc基因易位。随着c-myc蛋白表达水平的升高,c-myc基因易位的发生率也随之升高。结论 c-myc蛋白高表达与c-myc基因易位密切相关。c-myc蛋白可用于DLBCL中c-myc基因易位的筛选,建议对c-myc蛋白阳性细胞百分率40%者行FISH检测以进一步明确有无c-myc基因易位。
[Abstract]:Objective to explore the correlation between c-myc protein expression and c-myc gene translocation in order to find a suitable value for clinical c-myc protein. Methods Immunohistochemical method and fluorescence in situ hybridization in situ hybridization were used to determine the translocation status of c-myc gene. The diffuse large B-cell lymphoma was detected by fish in 293 cases of diffuse large B-cell lymphoma. The expression level of c-myc protein and the translocation status of c-myc gene in DLBCLs were analyzed. Results the correlation between c-myc protein expression and c-myc gene translocation was analyzed in 293 cases of DLBCL. The percentage of c-myc protein positive cells in 29 cases was 40%. The percentage of c-myc protein positive cells in 1 case was 40%. The percentage of c-myc protein positive cells was 40% and no c-myc gene translocation occurred. The expression level of c-myc protein increased with the increase of c-myc protein expression. The incidence of c-myc gene translocation also increased. Conclusion. The high expression of c-myc protein is closely related to c-myc gene translocation. C-myc protein can be used to screen c-myc gene translocation in DLBCL. It was suggested that the percentage of c-myc protein positive cells should be detected by FISH in order to determine the translocation of c-myc gene.
【作者单位】： 国家癌症中心/中国医学科学院北京协和医学院肿瘤医院病理科;
【基金】：首都临床特色应用研究(Z151100004015121) 中国癌症基金会北京希望马拉松基金(LC2013B40、LC2014L13)
【分类号】：R733.1
【正文快照】： 弥漫大B细胞淋巴瘤(diffuse large B-cell lym-phoma,DLBCL)是非霍奇金淋巴瘤(non-Hodgkin’slymphoma,NHL)最常见的病理亚型,DLBCL在西方国家占成人NHL的30%~40%[1,2],在我国等发展中国家所占比例更高,达60%[2]。近年来,虽然利妥昔单抗(Rituximab)的广泛应用明显提高DLBCL的，

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