大豆种质SX6907抗锈性状遗传及基因定位研究

发布时间：2018-01-29 01:15

本文关键词： 大豆锈病抗锈遗传基因定位　出处：《中国农业科学院》2016年硕士论文　论文类型：学位论文

【摘要】：由豆薯层锈菌(Phakopsora pachyrhizi)引起的大豆锈病,已经成为世界大豆主产区主要病害,严重影响大豆产量。与化学药物防治相比,培育抗锈品种是控制大豆锈病最为安全有效的途径。抗锈资源筛选、抗锈基因遗传分析是开展抗病育种和抗病机理研究的基础。本研究在前期发现一份对锈菌小种SS4表现高抗的大豆资源SX6907的基础上,开展SX6907抗锈遗传分析及抗锈基因定位研究。主要研究结果如下:1.抗锈遗传分析:利用感病品种天隆1号、中豆40及蒲豆11分别与抗锈亲本SX6907杂交,构建了三个F2群体。采用离体叶片接种法,对亲本以及三个组合的F1和F2单株进行抗锈鉴定。结果表明:三个组合的F1植株都表现不同于双亲的的红黑色(Red-black,RB)病斑反应型;F2群体中出现3种抗病反应型,经卡方测验,三种抗病反应型分离比例符合1免疫(Immune,IM):2红黑色病斑(RB):1黄褐色病斑(TAN)的分离比,说明抗锈资源SX6907对锈菌小种SS4的抗性可能是由一对不完全显性基因控制。2.抗锈基因初步定位:利用BSA(Bulked Segregation Analysis)法,对抗、感亲本(中豆40、天隆一号、蒲豆11和SX6907)及抗、感池进行多态性筛选及标记连锁分析,结果表明18号染色体上、Rpp1位点附近标记与抗锈性状连锁。通过对Rpp1位点附近标记在中豆40×SX6907组合F2群体上的标记分析,将抗锈基因初步定位在标记BARCSOYSSR_18_1856和BARCSOYSSR_18_1864之间,物理距离约为363.9kb,标记分离比例与3种抗病反应分离比例一致。利用天隆1号×SX6907和蒲豆11×SX6907两个组合的F2分离群体验证了该结果。3.抗锈基因精细定位:进一步在初定位区间内开发出多态性SSR标记11个。利用初步定位侧翼标记对天隆1号×SX6907杂交获得的另外800株F2单株进行重组子筛选,获得重组单株16株。采用新开发的标记对重组子进行标记分析,结合表型鉴定,最终将抗锈基因定位在标记SSR24和SSR40之间,物理距离约为111.9Kb。与已知Rpp1资源的抗锈基因定位区间和抗性表现进行比较,推测SX6907抗锈基因为不同于Rpp1位点的新基因,将其命名为Rpp6907。4.抗锈候选基因分析:对Rpp6907所在定位区间内基因预测显示,该区间存在11个候选基因。其中三个基因Glyma18g51930、Glyma18g51950和Glyma18g51960属于NBS-LRR类抗病基因家族,推测它们可能是Rpp6907位点的抗锈候选基因。
[Abstract]:Soybean rust caused by Phakopsora pachyrhizi) has become the main disease in soybean producing areas in the world. Compared with chemical drug control, breeding rust resistant varieties is the safest and effective way to control soybean rust. Genetic analysis of rust resistance genes is the basis of the research on resistance breeding and disease resistance mechanism. This study was based on the discovery of a soybean resource SX6907 with high resistance to rust race SS4. The main results of this study were as follows: 1. Genetic analysis of rust resistance: using the susceptible cultivar Tianlong 1. Three F2 populations were constructed by crossing Zhongdou 40 and Pudou 11 with rust resistant parent SX6907, respectively. The method of leaf inoculation in vitro was used. The rust resistance of the F1 and F2 plants of their parents and three combinations were identified. The results showed that all the F1 plants of the three combinations showed Red-black which was different from their parents. RBB); In F2 population, there were three resistant response types, and the segregation ratio of the three disease resistance response types was in accordance with 1 immunized Immune by chi-square test. The isolation ratio of IM):2, RBC: 1. It is suggested that the resistance of rust resistant resource SX6907 to SS4 may be controlled by a pair of incomplete dominant genes. Bulked Segregation Analysis. Antagonistic, susceptible parents (Zhongdou 40, Tianlong 1, Pudou 11 and SX6907) and resistance, susceptibility pool were screened by polymorphic analysis and marker linkage analysis. The results showed that chromosome 18 was on chromosome 18. Rpp1 locus markers were linked to rust resistance traits. The markers near Rpp1 locus were analyzed on F _ 2 population of Zhongdou 40 脳 SX6907 combination. The rust resistance gene was preliminarily located between the labeled BARCSOYSSR_18_1856 and BARCSOYSSR_18_1864, and the physical distance was about 363.9 kb. The segregation ratio of marker was the same as that of three disease resistance reactions. The results were verified by F2 population of Tianlong 1 脳 SX6907 and Pudou 11 脳 SX6907. 3. The antirust gene spermatozoa was obtained. Fine positioning:. Furthermore, 11 polymorphic SSR markers were developed in the initial locational region. The recombinant clones of another 800 F2 plants obtained from Tianlong 1 脳 SX6907 hybridization were screened by using the preliminary flanking marker. A total of 16 recombinant single strains were obtained. The newly developed markers were used for marker analysis and phenotypic identification. Finally, the rust resistance gene was located between SSR24 and SSR40. The physical distance is about 111.9 KB. Compared with the known Rpp1 resources, we speculated that the rust-resistant gene of SX6907 was different from the new gene at Rpp1 site. Rpp6907.4. Rpp6907.4. Rpp6907.4. Rpp6907.4.Analysis of rust resistance candidate genes: prediction of genes. There are 11 candidate genes in this region, three of which are Glyma18g51930. Glyma18g51950 and Glyma18g51960 belong to the family of NBS-LRR resistance genes, which may be the candidate genes of Rpp6907 locus.
【学位授予单位】：中国农业科学院
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：S435.651

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