木薯转录因子基因MeHDZ14的克隆与分析

发布时间：2018-01-29 09:25

本文关键词： 木薯 MeHDZ 结构特性表达分析　出处：《作物学报》2017年08期 　论文类型：期刊论文

【摘要】：HD-Zip家族基因在植物生长发育和逆境胁迫中起重要作用。为了研究MeHDZ14基因在非生物胁迫(尤其是干旱)应答中的作用,选用对干旱信号反应灵敏、相对耐旱的木薯品种"SC124"作为实验材料,利用RT-PCR克隆了MeHDZ14基因。生物信息学分析发现,MeHDZ14基因编码的蛋白具有典型的HD-Zip保守结构域。将该基因编码的蛋白与GFP融合,亚细胞MeHDZ14:GFP重组蛋白定位于细胞核。同时,酵母Y187中的转录自激活试验结果也表明,MeHDZ14蛋白具有明显转录自激活功能。推断MeHDZ14是一个典型的HD-Zip I转录因子。MeHDZ14启动子区具有多个ABA响应元件ABRE(ABA response element)。基因差异表达分析结果表明,MeHDZ14基因在叶片和根中的表达受干旱胁迫的诱导,并对外源ABA具有明显的响应。因此,认为MeHDZ14基因通过ABA依赖信号传导途径参与调控木薯干旱响应。此外,还发现MeHDZ14基因的编码区虽然存在数个SNP,但表现出高度保守性,且在不同木薯品种中的表达对干旱胁迫均有明显的响应,为进一步研究该基因的功能奠定了基础。
[Abstract]:HD-Zip family genes play an important role in plant growth and stress. In order to study the role of MeHDZ14 gene in abiotic stress (especially drought) response. Cassava variety "SC124", which is sensitive to drought signals and relatively drought tolerant, was used as experimental material. MeHDZ14 gene was cloned by RT-PCR. The protein encoded by MeHDZ14 gene has a typical conserved domain of HD-Zip. The protein encoded by this gene is fused with GFP. The recombination protein of subcellular MeHDZ14:GFP was located in the nucleus. At the same time, the transcriptional self-activation test in yeast Y187 also showed that. MeHDZ14 protein has the function of transcriptional self-activation. It is concluded that MeHDZ14 is a typical HD-Zip. The I transcription factor. MeHDZ14 promoter has multiple ABA response elements ABRE(. ABA response element.Gene differential expression analysis showed that. The expression of MeHDZ14 gene in leaves and roots was induced by drought stress and had obvious response to exogenous ABA. It is suggested that MeHDZ14 gene is involved in the regulation of cassava drought response through ABA dependent signal transduction pathway. In addition, it is found that there are several SNP in the coding region of MeHDZ14 gene. But it showed a high degree of conservatism, and the expression in different cassava varieties had obvious response to drought stress, which laid a foundation for further study of the function of the gene.
【作者单位】：中国热带农业科学院热带生物技术研究所/农业部热带作物生物学与遗传资源利用重点实验室;
【基金】：国际科技合作与交流项目(31561143012,2013DFA32020) 国家自然科学基金项目(31501378)资助~~
【分类号】：S533
【正文快照】： 本研究由国际科技合作与交流项目(31561143012,2013DFA32020)和国家自然科学基金项目(31501378)资助。This study was supported by the International ScienceTechnology Cooperation Program of China(31561143012,2013DFA32020)and theNational Natural Science Foundatio

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