猪流行性腹泻病毒LN-2015-1株分离鉴定及其S基因的变异分析

发布时间：2018-01-29 09:35

本文关键词： 猪流行性腹泻病毒辽宁分离株 S基因抗原表位变异分析　出处：《中国兽医学报》2017年08期 　论文类型：期刊论文

【摘要】：为了解辽宁猪流行性腹泻病毒(PEDV)来源及变异特性,经PCR诊断,细胞分离,细胞病变观察、RT-PCR以及S基因序列鉴定,成功分离1株PEDV毒株(LN-2015-1)。S基因序列分析显示,与CV777毒株相比,除点突变外,分离株还出现最长9bp的插入,6bp的缺失和13bp连续突变。氨基酸也存在最长3aa插入和2aa缺失,同时存在4处连续3个及以上的氨基酸突变,抗原表位分析表明在5个表位区共发生16个氨基酸的突变。同源性分析显示与HB-HA2015毒株的同源关系最近,序列相似性为99.2%,与2010年以来国内外代表变异毒株同源性较高,达到98.5%~98.8%,而与2010年以前的传统同源性较低,在93.2%~95.6%之间;进化树分析显示该毒株与近年来国内外流行的变异毒株属于同一群,并且与HB-HA2015形成一小的分支,而与传统毒株进化距离较远。
[Abstract]:In order to understand the origin and variation characteristics of Liaoning porcine epidemic diarrhea virus (PEDVV), PCR diagnosis, cell isolation, cytopathic observation, RT-PCR and S gene sequence identification were performed. The sequence analysis of LN-2015-1N. S gene of one PEDV strain showed that, in addition to the point mutation, the longest 9 BP insertion was found in the isolated strain compared with the CV777 strain. 6 BP deletion and 13 BP continuous mutation. The amino acid also had the longest 3 AA insertion and 2 AA deletion, and there were 4 consecutive 3 or more amino acid mutations. The antigenic epitope analysis showed that there were 16 amino acid mutations in 5 epitopes. Homology analysis showed that the homology with HB-HA2015 strain was the closest, the sequence similarity was 99.2%. The homology was higher than that of domestic and foreign representative mutant strains since 2010, reaching 98.5% and 98.8%, but lower than that of traditional strains before 2010 (93.2% or 95.6%). The phylogenetic tree analysis showed that the strain belonged to the same group as the mutant strain that had been prevalent in recent years, and formed a small branch with HB-HA2015, but it was far from the traditional strain.
【作者单位】：锦州医科大学畜牧兽医学院;中国科学院微生物研究所;辽宁省动物卫生监测预警中心;营口市动物疫病预防控制中心;
【基金】：辽宁省自然科学基金资助项目(2015010779) 辽宁省百千万人才工程资助项目(2014921012)
【分类号】：S852.651
【正文快照】： 猪流行性腹泻(porcine　epidemic　diarrhea,加入双抗使终浓度青霉素100 U/mL、链霉素PED)是由猪流行性腹泻病毒(porcine　epidemic　di-100mg/L,经0.22μm滤膜过滤,-80℃保存备用。arrhea　virus,PEDV)引起的以腹泻、呕吐、脱水和对1.2　主要材料　Trans　2KDNA　Marker、Tra

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