谷子类胡萝卜素生物合成途径SiLCYB基因与米色形成的关系

发布时间：2018-01-30 04:56

本文关键词： β-胡萝卜素米色 SiLCYB 籽粒灌浆表达模式　出处：《分子植物育种》2016年06期 　论文类型：期刊论文

【摘要】：谷子是中国北方重要杂粮作物,米色是评价谷子品质的重要指标,目前关于谷子米色的形成机制仍不明确。本研究选用米色分别为深黄、浅黄、白色和绿色的谷子品种各2个,对这8个谷子品种进行了总类胡萝卜素含量、β-胡萝卜素含量与米色差异间关系的分析,以及分子水平4个β-胡萝卜素代谢相关基因在籽粒不同灌浆阶段的表达模式分析,结果表明:CCI指数作为米色测定的综合指标,可对不同品种米色差异进行鉴定,且分别与总类胡萝卜素、β-胡萝卜素含量呈显著与极显著正相关。通过对8个品种SiLCYB基因组DNA克隆发现,只有深黄品种JG21中出现序列变异,有两个SNP位点,且第二处单核苷酸变异使相应氨基酸由谷氨酰胺变为精氨酸。SiLCYB表达不具有组织特异性,在叶中表达最高,茎中最低。通过对籽粒不同灌浆阶段β-胡萝卜素合成基因SiLCYB与另外3个β-胡萝卜素代谢相关基因(SiLCYBLCYE,SiHYD,SiCCD1)的表达分析发现:SiLCYB在大多数品种中表达基本恒定,且与β-胡萝卜素积累没有表现出显著相关性;而SiLCYE在不同品种中普遍呈现出与SiLCYB同增同减的表达模式,但表达水平低于SiLCYB;同时发现2个降解相关基因(SiHYD,SiCCD1)的表达与β-胡萝卜素积累在灌浆特定阶段表现出了显著或极显著负相关。因此,推测SiLCYB与降解基因SiHYD和SiCCD1共同作用,通过影响β-胡萝卜素和总类胡萝卜素在籽粒中的积累,进而影响米色的形成。
[Abstract]:Millet is an important crop in northern China and beige is an important index to evaluate the quality of millet. At present the formation mechanism of millet beige is still unclear. In this study the beige color is dark yellow and light yellow respectively. The relationship between total carotenoid content, 尾 -carotene content and beige color difference was analyzed in two white and two green millet varieties. The expression patterns of four 尾 -carotene metabolism-related genes at molecular level were analyzed at different grain filling stages. The results showed that the proportion of CCI was a comprehensive index for beige determination. The difference of beige color of different varieties could be identified, and there was a significant and extremely significant positive correlation with total carotenoid and 尾 -carotene content, respectively. The genomic DNA cloning of eight varieties of SiLCYB was found. There were only two SNP loci in JG21. The second single nucleotide variation changed the corresponding amino acid from glutamine to arginine. SiLCYB expression was not tissue specific, and the highest expression was found in leaves. The content of 尾 -carotene biosynthesis gene SiLCYB and the other three 尾 -carotene metabolism-related genes (SiLCYBLCYEN SiHYD) in different grain filling stages were compared with those of three other genes related to 尾 -carotene metabolism. SICCD1) expression analysis showed that the expression of 1% SiLCYB was almost constant in most cultivars and had no significant correlation with 尾 -carotene accumulation. The expression pattern of SiLCYE was similar to that of SiLCYB, but the expression level was lower than that of SiLCYB. At the same time, it was found that the expression of two degradation related genes (SiHYDX) and 尾 -carotene accumulation was significantly or extremely negatively correlated with 尾 -carotene accumulation at the specific filling stage. Therefore, there was a significant negative correlation between 尾 -carotene accumulation and 尾 -carotene accumulation. It is inferred that the interaction of SiLCYB with SiHYD and SiCCD1 could affect the accumulation of 尾 -carotene and total carotenoid in grains, and then affect the formation of beige.
【作者单位】：山西农业大学农学院;农业部黄土高原作物基因资源与种质创制重点实验室;杂粮种质资源发掘与遗传改良山西省重点实验室;山西农业大学农业生物工程研究所;
【基金】：国家自然科学基金(31371693;31471556;31471502) 教育部高等学校博士学科点专项科研基金(20131403110001) 山西农业大学科技创新基金(20142-07)共同资助
【分类号】：S515
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