柴达木盆地梭梭CMO基因的克隆及其蛋白结构预测

发布时间：2018-01-30 06:45

本文关键词： 梭梭 CMO基因序列分析蛋白结构　出处：《西北林学院学报》2016年02期 　论文类型：期刊论文

【摘要】：对柴达木盆地梭梭的CMO基因进行扩增,并利用生物软件对CMO基因序列进行分析、对蛋白结构进行预测。结果表明,柴达木盆地梭梭CMO基因长度为1 341bp,编码447个氨基酸。CMO蛋白亲水性、二级结构、亚细胞定位、信号肽、跨膜螺旋区、三级结构预测结果显示柴达木盆地梭梭CMO蛋白亚细胞定位于叶绿体,无信号肽,蛋白二级结构主要为无规则卷曲。CMO蛋白具有多个多种类型的功能位点,为蛋白功能的实现提供了保障。此外CMO蛋白的亲水性较强,无跨膜螺旋区。柴达木盆地梭梭CMO蛋白三维结构预测结果显示CMO蛋白三维结构由α螺旋、β折叠和无规则卷曲互相盘绕而成。
[Abstract]:The CMO gene of Haloxylon ammodendron in Chaidamurum Basin was amplified, and the sequence of CMO gene was analyzed by using biological software, and the protein structure was predicted. The length of CMO gene of Haloxylon ammodendron in Chaidamu Basin was 1341 BP, encoding 447 amino acid. CMO protein hydrophilic, secondary structure, subcellular localization, signal peptide, transmembrane helical region. The results of tertiary structure prediction showed that the CMO subcells of Haloxylon ammodendron in Chaidamu Basin were located in chloroplast and had no signal peptide. The protein secondary structure is mainly irregular curl. CMO protein has many kinds of functional sites, which provides a guarantee for the realization of protein function. In addition, CMO protein has strong hydrophilicity. The prediction results of three-dimensional structure of CMO protein of Haloxylon ammodendron in Chaidamu Basin show that the three-dimensional structure of CMO protein is composed of 伪 helix, 尾 folding and irregular coiling.
【作者单位】：青海大学农牧学院;
【基金】：青海省科技厅自然科学基金项目(2012-Z-938Q) “123高层次人才培养工程”项目
【分类号】：S792.99
【正文快照】： 梭梭(Haloxylon ammodendron)为藜科(Che-nopodiaceae)梭梭属(Haloxylon Bunge)多年生小乔木,主要分布在我国新疆、内蒙古、甘肃、青海的半荒漠和荒漠地区。梭梭具有很强的抗旱性[1],是柴达木盆地旱生、盐生荒漠植被组成的主体,也是当地流动沙地治理的先锋树种[2]。在众多的渗

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