小桐子JcYkt6基因的克隆和表达分析

发布时间：2018-01-30 19:46

本文关键词： 小桐子 SNAREs JcYkt 克隆生物信息学分析表达分析　出处：《植物生理学报》2017年04期 　论文类型：期刊论文

【摘要】：以小桐子(Jatropha curcas)c DNA为模版,克隆了Jc Ykt6基因的CDS序列。序列分析表明该基因包含600 bp完全阅读框(ORF),编码199个氨基酸。预测其编码蛋白质的分子量为22.53 k Da,等电点为6.90。Blast搜索结果及进化分析结果表明,Jc Ykt6蛋白与香瓜和黄瓜的Ykt6蛋白序列一致性最高(91%),亲缘关系最近。该基因编码的蛋白具有一个典型的VAMP基元。定量分析结果显示,Jc Ykt6基因在小桐子根、茎、叶、花、果皮和种子中都有表达,并且在种子中表达量最高。在种子生长发育的前期其表达水平不断增加,在种子生长发育的后期(50 d)达到最高值,然后下降。表达量变化过程与种子中储藏蛋白质合成趋势基本一致,推测其参与小桐子种子储藏蛋白质的合成和运输。ABA、PEG、Na Cl、4°C和机械损伤处理下Jc Ykt6表达水平都不同程度上调,推测Jc Ykt6具有多种功能,可能参与小桐子非生物胁迫响应和激素信号传导。
[Abstract]:Jatropha curcas)c DNA was used as template. The CDS sequence of JC Ykt6 gene was cloned. The sequence analysis showed that the gene contained 600bp complete reading frame (ORF). The predicted molecular weight of the encoded protein was 22.53 kDa, and the isoelectric point was 6.90. Blast search results and evolutionary analysis. JC Ykt6 protein had the highest consistency with Ykt6 protein sequence of muskmelon and cucumber. The result of quantitative analysis showed that JC Ykt6 gene was expressed in root, stem, leaf, flower, pericarp and seed of Tung Tung. The expression level increased continuously at the early stage of seed growth and development, and reached the highest value at the later stage of seed growth and development. The process of expression change was basically consistent with the trend of protein synthesis in seed storage. It was speculated that it was involved in the synthesis and transport of stored protein in jatropha jatropha seed. The expression of JC Ykt6 was up-regulated at 4 掳C and mechanical injury, suggesting that JC Ykt6 had many functions and might be involved in abiotic stress response and hormone signal transduction.
【作者单位】：昆明理工大学现代农业工程学院;云南农业大学园林园艺学院;云南师范大学生命科学学院;昆明理工大学建筑与城市规划学院;
【基金】：国家自然科学基金(31460355、31260064和31460059)~~
【分类号】：Q943.2;S794.9
【正文快照】： 收稿2016-11-09修定2017-03-14真核细胞含有复杂的内膜系统,细胞内的物质交换通过囊泡运输方式来完成(Staehelin 1997)。囊泡运输主要包括运输囊泡的出芽、定向移动、锚定和膜融合等过程,可溶性N-乙基马来酰亚胺敏感因子附着蛋白受体(SNAREs)在膜融合过程中发挥重要作用(Cai等

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