中国汉族人群PDE4D基因多态性与强迫症的关联研究

发布时间：2018-01-30 22:41

本文关键词： 强迫障碍磷酸二酯酶4D 单核苷酸多态性　出处：《青岛大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:探索中国汉族人群磷酸二酯酶4D(PDE4D)基因多态性与强迫症可能存在的关系,寻找与强迫症相关的生物遗传标志。方法:依据国际疾病与相关健康问题统计分类第十版(ICD-10)关于强迫症的诊断标准,先用聚合酶链式反应(PCR)扩增目的基因的片段,然后应用限制性内切酶片段长度多态性(RFLP)来分析测定序列的差异性,即采用PCR-RFLP技术,测定符合纳入标准的共计859例受试者的PDE4D基因rs1838733位点的基因型、等位基因的频率分布,其中包含400例强迫症患者,459例健康对照者;分析此位点与强迫症的关联性。依据发病特点,按性别、发病年龄分层后,比较分层后各组间该位点的基因型频率、等位基因频率分布与强迫症的关联性。结果:经H-W检验,磷酸二酯酶4D基因rs1838733位点符合Hardy-Weinderg平衡定律(p0.05),该位点共检出3种基因型,分别为AA、AG、GG,通过对此位点基因型频率、等位基因频率分布比较,差异无显著性(P0.05);依照性别、发病年龄分层后,该位点在分组后组与组之间上的基因型频率、等位基因频率分布差异亦均无显著性(P0.05)。结论:在中国汉族人群中,磷酸二酯酶4D(PDE4D)基因rs1838733位点的单核苷多态性未发现与强迫症的发病存在关联性,可能不是有标记意义的位点。
[Abstract]:Objective: to explore the relationship between phosphodiesterase 4DG PDE4D gene polymorphism and obsessive-compulsive disorder (OCD) in Chinese Han population. Methods: the diagnostic criteria for obsessive-compulsive disorder (OCD) according to International Statistical Classification of Disease and related Health (ICD-10) (10th) were searched for the biomarkers associated with obsessive-compulsive disorder (OCD). Polymerase chain reaction (PCR) was used to amplify the fragment of the target gene, and then restriction endonuclease fragment length polymorphism (RFLP) was used to analyze the sequence difference. The genotypes and alleles of rs1838733 loci of PDE4D gene were determined by PCR-RFLP technique in a total of 859 subjects who met the inclusion criteria. It included 400 patients with obsessive-compulsive disorder (OCD) and 459 healthy controls. The relationship between this locus and obsessive-compulsive disorder (OCD) was analyzed. According to the characteristics of the disease, the genotype frequency of the locus was compared according to sex and age. Relationship between allele frequency distribution and obsessive-compulsive disorder. Results: H-W test. The rs1838733 locus of phosphodiesterase 4D gene was in accordance with the Hardy-Weinderg equilibrium law (p0.05A). GG, there was no significant difference in genotype frequency and allele frequency distribution of this locus (P 0.05). According to sex and age stratification, there was no significant difference in genotype frequency and allele frequency distribution between the two groups. Conclusion: in Chinese Han population, there is no significant difference in genotype frequency and allele frequency distribution. The single nucleoside polymorphism of the rs1838733 locus of phosphodiesterase 4D / PDE4D gene was not associated with the onset of obsessive-compulsive disorder (OCD) and might not be a marker site.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R749.7

【参考文献】