低温胁迫下蒙古黄芪差异表达基因分析

发布时间：2018-01-31 01:49

本文关键词： 蒙古黄芪低温胁迫异黄酮植物内源激素差异表达基因　出处：《植物生理学报》2017年04期 　论文类型：期刊论文

【摘要】：温度是调控植物生长发育和代谢产物积累的重要因素。为了研究温度调控蒙古黄芪次生代谢产物积累的分子机制,本文基于转录组测序结果对常温和低温组的蒙古黄芪幼苗基因表达水平进行对比分析。结果表明:相对于常温组,低温组差异表达基因共3 896个,其中表达上调和下调的基因数目分别为2 420和1 476个。关键基因差异表达分析显示,低温组异黄酮代谢途径中大部分关键基因表达量都显著上调,而植物激素合成转导途径中的关键基因表达模式各有不同。由此可见,低温胁迫能够促进蒙古黄芪中部分药用成分的合成,同时能改变部分内源植物激素的合成和转导,从而使其更好的适应低温环境。
[Abstract]:In order to study the molecular mechanism of temperature regulating the accumulation of secondary metabolites of Astragalus mongolicus (Astragalus mongolicus), temperature is an important factor in regulating plant growth and metabolite accumulation. Based on the results of transcriptome sequencing, the gene expression levels of Astragalus mongolicus seedlings at room temperature and low temperature were compared. The results showed that there were 3 896 differentially expressed genes in low temperature group compared with normal temperature group. The number of genes up-regulated and down-regulated were 2 420 and 1 476 respectively. The differential expression analysis of key genes showed that most of the key genes in isoflavone metabolism pathway were significantly up-regulated in hypothermia group. However, there are different expression patterns of key genes in plant hormone synthesis transduction pathway. Therefore, low temperature stress can promote the synthesis of some medicinal components in Astragalus mongolicus (Astragalus mongolicus). At the same time, it can change the synthesis and transduction of some endogenous plant hormones, so that it can adapt to low temperature environment better.
【作者单位】：复旦大学生命科学学院;
【基金】：国家自然科学基金(8137963)~~
【分类号】：S567.239
【正文快照】： 收稿2016-12-26修定2017-03-10蒙古黄芪[Astragalus membranaceus Bge.var.mongolicus(Bge.)Hsiao]是我国最重要的中药材之一,有广泛的生理和药理活性,能增强机体免疫力,清除自由基,在保护心肌细胞、增强心肌活力、抗肿瘤等方面有广泛应用(陈国辉和黄文凤2008)。黄芪皂苷(主要

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