基于RNA-seq数据的差异基因和异构体检测

发布时间：2018-01-31 02:52

本文关键词： RNA-seq 差异基因差异异构体负二项分布 exact test　出处：《南京大学学报(自然科学)》2016年02期 　论文类型：期刊论文

【摘要】：基因和异构体表达水平的差异检测是获取基因和异构体功能的重要途径,目前差异检测已经是转录组研究中一个重要的研究方向.RNA-seq技术近年来被广泛用于差异基因的检测.为模拟读段的非均匀分布,通常采用负二项分布对读段计数进行建模.现存的负二项分布模型大都是直接对基因读段计数进行建模,不能进行差异异构体检测.提出基于PGseq模型计算出的基因和异构体表达水平的负二项分布模型,采用exact test方法进行差异分析,解决了异构体的差异检测的问题.经实验验证,该方法在基因和异构体两方面的差异检测中都具有较高的准确度和灵敏度.
[Abstract]:The differential detection of gene and isomer expression level is an important way to obtain the function of gene and isomer. At present, differential detection has been an important research direction in transcriptome research. RNA-seq technology has been widely used in the detection of differential genes in recent years. Usually the negative binomial distribution is used to model the reading segment count. Most of the existing negative binomial distribution models are used to model the gene reading segment count directly. The negative binomial distribution model of gene and isomer expression level calculated by PGseq model was put forward, and the difference analysis was carried out by exact test method. The problem of differential detection of isomers has been solved. The experimental results show that the method has high accuracy and sensitivity in gene and isomer differential detection.
【作者单位】：南京航空航天大学计算机科学与技术学院;
【基金】：国家自然科学基金(61170152) 中央高校基本科研业务费专项(CXZZ11_0217)
【分类号】：Q78
【正文快照】： RNA-seq即高通量RNA测序,与传统基因芯片技术相比,具有信噪比高,成本低,测序速度快,应用范围广等优势[1].RNA-seq技术一次测序实验可产生数以千万计的读段(read)数据,其核心思想是通过将读段数据映射(mapping)定位到参考基因组或转录组上得到其量化的表达值[2].RNA-seq技术可

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