两个耐贮性不同的红肉苹果株系果实硬度与香气成分及相关酶活性与基因表达差异分析

发布时间：2018-01-31 07:01

本文关键词： 苹果红肉香气成分硬度基因表达酶活性　出处：《园艺学报》2017年02期 　论文类型：期刊论文

【摘要】：以‘红心7号’和‘红心9号’两个红肉苹果新品系的成熟果实为试材,检测贮藏期间硬度与香气成分及其相关酶活性与基因表达量。‘红心9号’苹果贮藏期间果实硬度极显著低于‘红心7号’,而乙烯释放速率极显著高于‘红心7号’;总香气物质、酯类含量及AAT1、AAT2和LOX基因表达量均极显著高于‘红心7号’,而在贮藏后期(90~120 d)果实醇类与醛类含量及HPL和ADH基因表达量均极显著低于‘红心7号’;PG、XET、PME、AM、α-L-Af和β-Gal等6个果实软化相关基因表达量及其酶活性大都极显著高于‘红心7号’。上述结果表明乙烯释放速率和酯类含量高及酯类生物合成和果实软化相关基因上调表达可能是导致‘红心9号’苹果贮藏期间果实硬度显著低于‘红心7号’的主要原因。香气物质种类、含量及其变化能作为果实贮藏品质评价的指标之一。
[Abstract]:The mature fruits of two new red apple lines, 'Hongxin 7' and 'Hongxin 9', were used as test materials. The firmness, aroma components, related enzyme activity and gene expression of 'Hongxin 9' apple during storage were significantly lower than that of 'Hongxin 7'. The ethylene release rate was significantly higher than that of 'Hongxin 7'. The total aroma substance, ester content and the expression of AAT1, AAT2 and LOX genes were significantly higher than that of 'Hongxin 7'. However, the contents of alcohols and aldehydes and the expression of HPL and ADH genes were significantly lower than those of 'Hongxin 7' at the late stage of storage. PG,XET,PME,AM. The expression and enzyme activity of 伪 -L-Af and 尾 -Gal related genes were significantly higher than that of 'Hongxin 7'. These results indicated that ethylene release rate and ester content were high and ester biosynthesis was high. The up-regulated expression of the genes related to fruit softening may be the main reason that the fruit firmness of 'Hongxin 9' apple was significantly lower than that of 'Hongxin 7' during storage. Content and its change can be used as one of the indexes of fruit storage quality evaluation.
【作者单位】：山东农业大学作物生物学国家重点实验室;
【基金】：国家公益性行业(农业)科研专项(201303093) 国家自然科学基金项目(31572091)
【分类号】：S661.1
【正文快照】： 苹果果实含有较多的人体容易吸收的游离多酚,在抗氧化、预防心脑血管疾病及抗肿瘤等方面均具有较好的作用(陈学森等,2010;许海峰等,2016)。中国是世界上最大的苹果资源、生产和消费大国,目前生产上的主栽品种是‘红富士’,约占70%,存在品种单一及生产成本高等诸多问题,苹果

【相似文献】