苋菜amaAG基因克隆与生物信息学分析

发布时间：2018-01-31 06:00

本文关键词： 苋菜 AG基因克隆开花生物信息学　出处：《江西农业大学学报》2017年01期 　论文类型：期刊论文

【摘要】：以苋菜种子为初始材料,通过离体培养获得苋菜各阶段试管苗,利用实验室构建的苋菜开花过程转录组数据库(NCBI SRA number:SRR924089,SSR924090,SRR924091,SRR924092),采用RT-PCR结合RACE技术进行ama AG基因全长c DNA序列克隆,然后对其进行生物信息学分析。结果表明,苋菜ama AG基因全长为1 123 bp,其中5'UTR 122 bp,3'UTR 243 bp,poly A尾长17 bp,并包含一个741 bp的开放阅读框,编码246个氨基酸。通过生物信息学分析可知,该蛋白属于亲水蛋白,但不是稳定蛋白;属于MADS和K-box超级家族,包含MADS_MEF2-like和K-box功能位点,与植物开花相关。ama AG基因差异表达分析表明,该基因是在开花期表达量最高。说明本研究已获得了苋菜ama AG基因,这为将来深入研究其功能奠定了基础,也为研究苋菜开花分子机制提供参考。
[Abstract]:Using amaranth seed as the initial material, the in vitro culture of amaranth in vitro was used to obtain the in vitro plantlets of amaranth. SRA number: SRR924089 and SSR924090, SRR924091, were used to construct a transcriptional database of amaranth. The full-length c DNA sequence of ama AG gene was cloned by RT-PCR combined with RACE technique and then analyzed by bioinformatics. The total length of ama AG gene in amaranth is 1 123 BP, of which 5 UTR122 bp1 3 UTR243 BP 3 poly A tail length is 17 BP. It contains an open reading frame of 741 BP and encodes 246 amino acids. Bioinformatics analysis shows that the protein belongs to hydrophilic protein, but it is not a stable protein. It belongs to MADS and K-box superfamily and contains MADS_MEF2-like and K-box functional sites. The results indicated that the ama AG gene had been obtained in this study, which laid a foundation for further study of its function and provided a reference for studying the molecular mechanism of amaranth flowering.
【作者单位】：福建农林大学园艺植物生物工程研究所;
【基金】：福建省重大科技专项(2015NZ0002) 高等学校博士学科点专项新教师类科研基金(20123515120009) 福建省自然科学基金(2013J05045)~~
【分类号】：S636.4;Q943.2
【正文快照】： Project supported by National Natural Science Foundation of China:Fujian Major Scientific and TechnologicalProject(2015NZ0002),the Specialized Research Fund for the Doctoral Program New Teacher Kind of Higher Ed-ucation(20123515120009)and Fujian Natural

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