己烯雌酚影响新生小鼠睾丸引带相关基因表达的初步研究

发布时间：2018-01-31 05:54

本文关键词： 己烯雌酚睾丸引带雌激素受体雄激素受体增殖细胞核抗原肌动蛋白α 小鼠　出处：《中华男科学杂志》2017年07期 　论文类型：期刊论文

【摘要】：目的:通过不同剂量己烯雌酚(DES)孕期染毒,了解其对新生仔鼠睾丸引带中雄激素受体(AR)、雌激素受体α(ERα)、增殖细胞核抗原(PCNA)和肌动蛋白α1(ACTα1)mRNA表达的影响,以期窥探DES影响睾丸引带的作用途径。方法:将发现雌性昆明小鼠阴道栓当天记为孕第0天(GD0),在GD9~GD17分别给予不同剂量DES[0.02、0.1、0.5、10、50μg/(kg·d)]作为实验组,给予等体积二甲基亚砜及生理盐水分别作为溶剂和空白对照组。生后第1天处死雄性仔鼠获取睾丸引带,通过RT-PCR检测AR、ERα、PCNA和ACTα1 mRNA的表达量。结果:随着DES浓度的增加,ERαmRNA的表达逐渐增加,各实验组与溶剂对照组比较差异有统计学意义(RE~2=0.825,P0.05);AR、PCNA和ACTα1 mRNA的表达都呈下降趋势,高剂量组[10、50μg/(kg·d)DES组]与溶剂对照组比较差异有统计学意义(RA~2=0.713,RP~2=0.946,RT2=0.960,P0.01)。结论:正常新生昆明小鼠睾丸引带中存在ERα、AR、PCNA及ACTα1 mRNA的表达。孕期不同剂量DES染毒对新生小鼠睾丸引带细胞受体蛋白(ERα和AR)及功能蛋白(PCNA和ACTα1)mRNA表达的影响不同。外源性雌激素可能通过影响ER和或AR的代谢而影响睾丸引带细胞的增殖和收缩等功能,从而影响睾丸甚至整个雄性生殖系统的正常发育。
[Abstract]:Objective: to investigate the effects of different doses of diethylstilbestrol (DES) on androgen receptor (AR) and estrogen receptor (伪 -ER 伪) in the testicular region of newborn rats. The expression of proliferating cell nuclear antigen (PCNA) and actin 伪 1 (ACT 伪 1 1) mRNA was observed. Methods: the vaginal suppository of female Kunming mice was recorded as GD0 on day 0 of gestation and given different doses of DES in GD9~GD17. [(0. 02) 0. 1 0. 5 渭 g / kg 路d ~ (50 渭 g / kg 路d) were used as experimental group (P < 0. 05). Dimethyl sulfoxide (DMSO) and normal saline were given as solvent and blank control group respectively. On the first day after birth, male rats were killed to obtain testicular distraction band, and Arrow ER 伪 was detected by RT-PCR. Results: with the increase of DES concentration, the expression of ER 伪 mRNA increased gradually. The difference between the experimental group and the solvent control group was statistically significant (P 0.05). The expression of PCNA and ACT 伪 1 mRNA decreased in the high dose group. [There was significant difference between 10 渭 g / kg / d des group and solvent control group. Conclusion: there is ER 伪 AR in the testicular region of normal newborn Kunming mice. Expression of PCNA and ACT 伪 1 mRNA. Different doses of DES during pregnancy on the expression of ER 伪 and ER 伪 and ACT 伪 1 in Neonatal mice testicular Passage cells. The effect of mRNA expression is different. Exogenous estrogen may affect the proliferation and contraction of testicular elicitor cells by affecting the metabolism of ER and AR. This affects the normal development of the testis and even the entire male reproductive system.
【作者单位】：南方医科大学附属佛山市妇幼保健院小儿外科;深圳大学妇幼保健院小儿外科;汕头大学医学院第二附属医院小儿外科;
【基金】：国家自然科学基金(81501240) 深圳市科创委科研项目(JCYJ20150402092905161) 深圳市坪山新区卫生科研项目(201536)~~
【分类号】：R114
【正文快照】： 流行病学研究发现,孕期环境雌激素(environ-分成7组,分别为空白对照组(仅给予等体积生理mental estrogens,EEs)暴露与日益增加的男性先天盐水)、溶剂对照组[仅给予0.2 ml溶剂二甲基亚砜性生殖系统畸形及成年后的生殖、性功能异常,生殖(DMSO)]和5组实验组[分别给予DES剂量为:系

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