两株水貂阿留申全基因重组核酸疫苗的免疫效果初步评估

发布时间：2018-01-31 09:15

本文关键词： 水貂阿留申病核酸疫苗免疫抗原抗体复合物 γ球蛋白淋巴细胞　出处：《中国兽药杂志》2017年12期 　论文类型：期刊论文

【摘要】：为研究实验室构建的两株水貂阿留申重组核酸疫苗(pcDNA3.1-ADV-428和pcDNA3.1-ADV-428-487)对水貂的免疫原性,将其经肌肉注射接种到水貂体内进行免疫,免疫完成后对水貂进行攻毒,然后每隔两周对水貂进行指尖采血。应用流式细胞术检测全血中的CD8+淋巴细胞亚群,用间接ELISA法检测血清中的抗体水平,用血清蛋白电泳检测血清中γ球蛋白占总蛋白的百分比以及应用聚乙二醇沉淀比浊法检测水貂血清中抗原抗体复合物的含量,以对两株核酸疫苗的免疫效果进行初步评估。试验结果表明,pcDNA3.1-ADV-428和pcDNA3.1-ADV-428-487的各项数据都优于对照组,且pcDNA3.1-ADV-428-487的保护效果优于pcDNA3.1-ADV-428,两种核酸疫苗都展现了良好的疫苗潜质。
[Abstract]:To study the immunogenicity of mink recombinant nucleic acid vaccine pcDNA3.1-ADV-428 and pcDNA3.1-ADV-428-487. It was injected intramuscularly into mink to be immunized, and the mink was inoculated with the virus after immunization. The blood samples were collected from the fingertips of minks every two weeks. The CD8 lymphocyte subsets in the whole blood were detected by flow cytometry and the antibody levels in serum were detected by indirect ELISA method. The percentage of 纬 globulin to total protein in serum was detected by serum protein electrophoresis and the content of antigen-antibody complex in mink serum was detected by polyethylene glycol precipitation turbidimetry. The immunological effects of two nucleic acid vaccines were preliminarily evaluated. The data of pcDNA3.1-ADV-428 and pcDNA3.1-ADV-428-487 were superior to those of the control group. The protective effect of pcDNA3.1-ADV-428-487 was better than that of pcDNA3.1-ADV-428. Both nucleic acid vaccines showed good vaccine potential.
【作者单位】：吉林农业大学动物科学与技术学院;吉林农业科技学院;吉林农业大学研究生院;
【基金】：国家自然基金(31372436) 吉林省科技厅科技支撑计划(20160209006YY)
【分类号】：S852.4
【正文快照】： Preliminary Immune Effect Evaluation of Two ADV RecombinantDNA VaccinesSUN Li-jie1,LIU Dong-xu2,LI Jian-ming1,LENG Xue1,SHI Kun1,LI Dong1,DU Rui3*(1.College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China;2.Jilin Agr

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