基于芯片数据的神经分化基因网络互作分析为神经系统疾病的细胞疗法提供基础（英文）

发布时间：2018-01-31 10:40

本文关键词： 神经分化骨髓间充质干细胞蛋白质相互作用网络差异基因　出处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2017年02期 　论文类型：期刊论文

【摘要】：目的:通过筛选差异基因,获得控制骨髓间充质干细胞向神经细胞分化及神经发育的中心基因,为治疗神经系统疾病提供参考。方法:从基因表达综合数据库(Gene Expression Omnibus database)中获得芯片数据,利用生物信息学软件筛选差异基因,并对差异基因进行GO功能富集、蛋白互作网络分析和中心基因分析。结论:通过分析,初步推测Nrcam、Sema3a、Mapk8、Dlg4、Slit1、Creb1、Ntrk2、Cntn2和Pax6等中心基因在调控骨髓间充质干细胞向神经细胞的分化中发挥重要作用;Dcx、Nrcam、Sema3a、Cntn2、Slit1、Ephb1和Pax6等中心基因在神经发育过程中发挥作用;Fgf2、Tgfβ1、Vegfa、Serpine1、Il6和Stat1等中心基因在抑制神经分化过程中发挥作用。
[Abstract]:Objective: to obtain the central gene to control the differentiation and neural development of bone marrow mesenchymal stem cells (BMSCs) by screening differentially expressed genes. Methods: microarray data were obtained from Gene Expression Omnibus database. The differential genes were screened by bioinformatics software, and the differential genes were enriched with go function, protein interaction networks and central genes were analyzed. Conclusion: through the analysis, we preliminarily speculate Nrcam. Sema3a,Mapk8,Dlg4,Slit1,Creb1,Ntrk2. Central genes such as Cntn2 and Pax6 play an important role in regulating the differentiation of bone marrow mesenchymal stem cells into neural cells. Cntn2Slit1, Ephb1 and Pax6 genes play a role in neurodevelopment. Fgf2tgf 尾 1, Vegfa1, Serpine 1, Stat1 and other central genes play an important role in the inhibition of neural differentiation.
【作者单位】： Department
【基金】：Project supported by the Key Project of Hebei North University(No.120177) the Science and Technology Research Project of Hebei Province Department Institutions of Higher Learning(No.Z2015047),China
【分类号】：R741
【正文快照】： 1 Introduction Neurological disorders are problems of the nervous system,which may be possible to treat by the transplantation and differentiation of stem cells to enhance nerve regeneration.Several studies have shown that bone mesenchymal stem cells(BM

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