沙柳TAC1基因克隆与生物信息学及组织表达特性分析

发布时间：2018-02-01 00:56

本文关键词： 沙柳 TAC1基因基因克隆定量PCR 组织特异性表达　出处：《内蒙古农业大学》2016年硕士论文　论文类型：学位论文

【摘要】：树木的分枝发育在植物形态构成中具有十分重要的地位,决定着整株树木的空间结构,并对光合效率、树木生物量及园林造景等方面具有重要的影响。沙柳(Salix psammophila)的分枝角度多样,可形成直立型、标准型及柱形等多种树木空间结构,是挖掘调控树木空间结构变化基因资源的良好材料。本研究以沙柳为材料,克隆了SpsTAC1(Tiller Angle Control 1)基因,进行了生物信息学分析,构建了进化树,并做了组织特异性表达分析。研究结果如下：1.沙柳TAC1基因的克隆及生物信息学分析：本研究利用同源克隆技术获得沙柳SpsTAC1基因,CDS序列全长819bp,编码272个氨基酸。蛋白分子量为65269.3Da,理论等电点为5.16,蛋白结构预测无跨膜结构,蛋白亚细胞定位预测位于细胞质中。2.不同物种TAC1蛋白质多序列比对及进化树分析表明沙柳与杞柳、毛果杨同源性较高,且共同具有五个保守区域。3. TAC1基因的组织特异性表达。定量qRT-PCR检测结果表明TAC1基因在沙柳的根、茎、叶片、顶芽、叶腋中均有表达,茎中表达最为丰富,其次是叶腋,而根中表达最低。研究结果表明克隆获得了沙柳SpsTAC1基因,该基因功能可能与分枝角度调控有重要关系,研究结果为进一步明确TAC1基因功能奠定了基础。
[Abstract]:The branching and development of trees plays an important role in plant morphological composition, which determines the spatial structure of the whole tree and its photosynthetic efficiency. The biomass of trees and landscaping play an important role. Salix psammophila has a variety of branching angles and can form an upright type. Standard and column-shaped spatial structures of trees are good materials to excavate and control the genetic resources of tree spatial structure changes. In this study, Salix willow was used as the material. SpsTAC1(Tiller Angle Control 1) gene was cloned, bioinformatics analysis was carried out, and an evolutionary tree was constructed. The results are as follows: 1. Cloning of TAC1 gene and bioinformatics analysis: in this study, SpsTAC1 gene was obtained by homologous cloning. The total length of CDS sequence was 819 BP, encoding 272 amino acids. The molecular weight of the protein was 65269.3 Da.The theoretical isoelectric point was 5.16.The protein structure was predicted without transmembrane structure. Protein subcellular location prediction was located in cytoplasm. Multiple sequence alignment of TAC1 proteins of different species and phylogenetic tree analysis showed that Salix willow had high homology with willow and poplar. The results of quantitative qRT-PCR analysis showed that TAC1 gene was expressed in root, stem, leaf and terminal bud of Salix willow. The most abundant expression was in the stem, followed by the axillary and the lowest in the root. The results showed that the SpsTAC1 gene was obtained by cloning. The function of this gene may be related to the regulation of branching angle. The results of this study lay a foundation for further clarifying the function of TAC1 gene.
【学位授予单位】：内蒙古农业大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：S793.9

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