RUNX3与CHFR基因甲基化提示胃癌癌前病变的研究

发布时间：2018-02-01 05:23

本文关键词： RUNX CHFR 胃癌萎缩性胃炎　出处：《基因组学与应用生物学》2017年04期 　论文类型：期刊论文

【摘要】：本研究通过比较胃癌与萎缩性胃炎的RUNX3及CHFR基因表达情况探讨其中的相关性。选取2014年1月至2016年7月前来浙江舟山群岛新区旅游与健康职业学院进行胃镜检查的137例慢性萎缩性胃炎患者作为研究对象,分为胃炎轻度组、胃炎中度组与胃炎重度组,并选取同期胃癌患者42例作为胃癌组。取胃炎患者的胃体与胃窦处的黏膜,胃癌患者的癌灶组织、癌旁组织与远端正常处组织进行DNA的提取,并进行比较。经比较后,胃炎重度组患者的RUNX3与CHFR基因甲基化阳性率分别为29.63%、37.04%,与胃癌组患者的正常组织处比较,有显著的统计学差异,与胃癌组癌灶组织处比较无差异;而胃炎重度组患者的蛋白表达有缺失情况发生,经Elisa检测后,蛋白表达量与胃癌组癌灶组织处比较无差异。严重萎缩性胃炎患者的RUNX3与CHFR基因甲基化可抑制抑癌基因的表达,而异常升高的阳性率会影响患者的病程进展,可认为RUNX3与CHFR基因甲基化对癌前病变进程具有临床诊断意义。
[Abstract]:This study was to investigate the correlation between RUNX3 and CHFR gene expression in gastric cancer and atrophic gastritis. Travel to Zhoushan Islands New area Zhejiang Province from January 2014 to July 2016. A total of 137 patients with chronic atrophic gastritis (CAG) who were examined by gastroscopy with the health vocational college were studied. It was divided into three groups: mild gastritis group, moderate gastritis group and severe gastritis group. 42 patients with gastric cancer were selected as gastric cancer group. The mucosa of gastric body and antrum and the cancerous tissue of gastric cancer were taken from gastritis patients. DNA was extracted from paracancerous tissues and distal normal tissues and compared. After comparison, the positive rates of RUNX3 and CHFR gene methylation in patients with severe gastritis were 29.63%, respectively. 37.04, compared with the normal tissue of patients with gastric cancer, there was significant statistical difference, and there was no difference with the cancer tissue of gastric cancer group. In the severe gastritis group, the expression of protein was absent, which was detected by Elisa. RUNX3 and CHFR gene methylation in patients with severe atrophic gastritis could inhibit the expression of tumor suppressor genes. The abnormal increase in the positive rate may affect the progression of the disease. It is suggested that methylation of RUNX3 and CHFR genes is of clinical significance in the diagnosis of precancerous lesions.
【作者单位】：浙江舟山群岛新区旅游与健康职业学院;
【分类号】：R735.2
【正文快照】： *通讯作者,dingxuan0712@163.comStudy on Methylation of RUNX3 and CHFR Gene in Precancerous Lesions ofGastric CancerWang Chongyu*Zhoushan TourismHealth College,Zhoushan,316111*Corresponding author,dingxuan0712@163.com前病变的研究,36(4):1301-1306)RUNX3 and

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