连梅提取液对湿热泻痢仔猪的血清抗氧化指标、胃肠调节酶及十二指肠凋亡基因表达的影响

发布时间：2018-02-02 06:35

本文关键词： 连梅提取液湿热泻痢仔猪抗氧化功能胃肠调节酶凋亡基因　出处：《畜牧兽医学报》2017年01期 　论文类型：期刊论文

【摘要】：为了探索连梅提取液对湿热泻痢仔猪的抗氧化功能、胃肠调节酶及十二指肠凋亡基因表达的影响,试验采用了人工模拟高湿高热的环境,给仔猪灌服大肠杆菌辅助致使泻痢,选择泻痢仔猪分为模型组、阳性药物组和连梅提取液高、中、低剂量组,以正常环境下正常饲喂的仔猪作为对照,通过检测仔猪血清抗氧化酶、胃肠调节酶、肝ATP酶以及十二指肠凋亡基因Caspase-3、-8、-9 mRNA转录变化以分析连梅提取液治疗泻痢仔猪的作用机制。结果显示,湿热泻痢仔猪经连梅提取液进行治疗后,血清T-SOD、GSH-Px活力升高,LDH、MDA含量减少,与模型组相比较差异极显著(P0.01);乙酰胆碱酯酶、淀粉酶、肝Na+-K+-ATPase和Ca2+-Mg2+-ATPase活力较模型组显著增强(P0.05);T3、T4和GH分泌量显著升高(P0.05),GC分泌量显著降低(P0.05);同时各药物组仔猪十二指肠凋亡基因得到了不同程度的恢复,Caspase-8 mRNA表达量只有中剂量组较模型组极显著降低至2.62倍(P0.01);Caspase-3mRNA表达中,高、中剂量组分别降至正常组的5.82、3.29倍,较模型组差异显著(P0.05)或极显著(P0.01);Caspase-9mRNA表达量均显著降低(P0.05),且降低量与药物浓度成正比。由此可知,连梅提取液可以通过增强湿热泻痢仔猪抗氧化损伤能力,改善胃肠道的消化吸收,促进生长激素的分泌和降低凋亡基因的表达而达到治疗仔猪湿热泻痢。
[Abstract]:In order to explore the effects of Lianmei extract on the antioxidant function, gastrointestinal regulatory enzyme and duodenal apoptotic gene expression in piglets with damp-heat diarrhea, artificial simulation of high humidity and high fever was used in the experiment. The piglets were divided into three groups: the model group, the positive drug group and the high, middle and low dose groups. The piglets fed in normal environment were used as the control. Serum antioxidant enzymes, gastrointestinal regulatory enzymes, liver ATP enzymes and duodenal apoptotic gene Caspase-3 were detected in piglets. The transcriptional changes of -9 mRNA were used to analyze the mechanism of Lianmei extract in treating diarrhea piglets. The results showed that the serum T-SOD of damp-heat diarrhea piglets was treated with Lianmei extract. The activity of GSH-Px increased and the content of GSH-Px decreased, compared with the model group, the difference was very significant (P 0.01). The activities of acetylcholinesterase, amylase, liver Na K ATPase and Ca2 mg 2 ATPase were significantly increased compared with the model group. The secretion of T3, T4 and GH increased significantly in P0. 05 and P0. 05 and decreased significantly in P0. 05. At the same time, the apoptotic genes of duodenum were recovered to different degrees in the piglets of different drug groups. The expression of Caspase-8 mRNA in middle dose group was significantly lower than that in model group to 2.62 times (P 0.01). The expression of Caspase-3mRNA in the high and middle dose groups decreased to 5.82 ~ 3.29 times of the normal group, which was significantly higher than that of the model group (P 0.05) or extremely significant (P 0.01). The expression of Caspase-9mRNA was significantly lower than that of P0.05P, and the decrease was directly proportional to the concentration of the drug. The extract of Lianmei can improve the ability of anti-oxidation damage, improve the digestion and absorption of gastrointestinal tract, promote the secretion of growth hormone and decrease the expression of apoptotic gene in the piglets by enhancing the ability of anti-oxidative damage, promoting the secretion of growth hormone and reducing the expression of apoptotic gene.
【作者单位】：重庆市畜牧科学院农业部养猪科学重点实验室;
【基金】：农业部公益性行业(农业)科研专项(201303040-06) 重庆市农发资金项目(15406) 重庆市基本科研业务费(15438)
【分类号】：S858.28
【正文快照】： 仔猪湿热泻痢是由湿邪和热邪齐聚动物体内,蕴蒸不化,伤及脏腑,耗损阴津,致使仔猪水湿运化失常而形成泻痢的综合病证,长期的湿热壅滞严重影响仔猪的生长发肓[1]。在我国南方地区和长江流域地区,夏季炎热多雨,形成高湿高热环境,容易引起仔猪湿热泻痢;目前在防治湿热泻痢方面,主

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