小桐子类固醇还原酶基因Jc5β-StR的克隆和表达分析

发布时间：2018-02-02 09:23

本文关键词： 小桐子 Jcβ-StR 克隆生物信息学分析表达分析　出处：《生物技术通报》2017年07期 　论文类型：期刊论文

【摘要】：以小桐子(Jatropha curcas L.)cDNA为模版,克隆了Jc5β-StR基因的CDS序列,并对其序列进行了生物信息学分析。序列分析表明该基因包含1 173 bp开放阅读框(ORF),编码390个氨基酸。预测其编码蛋白质的相对分子量为44.23 k D,理论等电点为5.22。Blast搜索结果及进化分析结果表明,Jc5β-StR与蓖麻5β-St R蛋白序列一致性最高(87%)且亲缘关系最近。Jc5β-StR是一个短链还原酶,该蛋白编码有一个短链还原酶家族(SDRs)的黄体酮5β还原酶(5β-POR),还含有SDRs的6个保守结构域。组织表达结果显示Jc5β-StR在小桐子根、茎、叶、花、果皮和种子中都有表达,在种子中表达量最高,且随种子发育过程表达量逐渐上升,在种子生长发育的后期(50 d)达到最高值后下降。非生物胁迫(PEG、Na Cl、低温和机械损伤)诱导下,Jc5β-StR的表达量都不同程度上调,推测其参与小桐子非生物胁迫响应。
[Abstract]:The CDS sequence of Jc5 尾 -Str gene was cloned by using Jatropha curcas L. curcas as template. The sequence analysis showed that the gene contained 1 173 BP open reading frame (ORF). It encodes 390 amino acids. The predicted molecular weight of the encoded protein is 44.23 KD, the theoretical isoelectric point is 5.22. Blast search results and evolution analysis results show. Jc5 尾 -StR and Ricinus castor 5 尾 -StR protein have the highest sequence consistency (87.10%) and have the closest relationship. Jc5 尾 -StR is a short-chain reductase. The protein encodes a short chain reductase family (SDRs) of progesterone 5 尾 reductase 5 尾 -POR). There were also 6 conserved domains of SDRs. The results of tissue expression showed that Jc5 尾 -StR was expressed in roots, stems, leaves, flowers, pericarp and seeds, and the highest expression was found in seeds. The expression level increased gradually with the seed development and decreased after reaching the highest value at the late stage of seed growth and development. Induced by abiotic stress PEGN Na Cl (low temperature and mechanical damage). The expression of Jc5 尾 -StR was up-regulated in varying degrees, which suggested that it was involved in the abiotic stress response of Paulownia cuspidata.
【作者单位】：昆明理工大学现代农业工程学院;昆明理工大学建筑与城市规划学院;云南农业大学园林园艺学院;云南师范大学生命科学院;
【基金】：国家自然科学基金项目(31460355)
【分类号】：Q943.2;S794.9
【正文快照】： 短链还原酶家族(Short-chain dehydrogenases/reductases,SDRs)是迄今为止发现的最古老、最大的蛋白质超家族之一,存在各种生物形式中,如古细菌,原核生物,真核生物和病毒等[1-2]。SDR家族成员在脂类、氨基酸、碳水化合物、激素类、异生物质代谢和氧化还原反应传感机制中发挥重

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