甲状腺乳头状癌相关致病基因及microRNAs的筛选和生物信息学分析

发布时间：2018-02-02 11:58

本文关键词： 甲状腺乳头状癌致病基因 microRNAs 生物信息学分析　出处：《基因组学与应用生物学》2017年11期 　论文类型：期刊论文

【摘要】：本研究利用美国国家生物技术信息中心(NCBI)基因表达综合数据库(GEO)下载获取甲状腺乳头状癌(papillary thyroid carcinoma,PTC)m RNA表达谱芯片(7例PTC样本VS 7例正常组样本,登录号为GSM85-215~GSM85228)及miro RNA高通量测序数据(3例PTC样本VS 3例正常组样本,GSM1388420~GSM138-8425)。通过Qlucore Omics Explorer(QOE)3.2、DAVID 6.7、STING 9.1、mi Records等分析软件对PTC差异基因及micro RNA进行综合生物信息学分析。筛选出来497个甲状腺乳头状癌相关致病基因及86个相关micro RNAs;其中,致病基因中上调表达的有257个,下调表达的有240个;micro RNAs中上调表达的有47个,下调表达的有39个。对其进行生物信息学分析发现,ERBB3、TNNT1、MYL1、POSTN基因及hsa-mi R-183-5p(ERBB4)、hsa-mi R-139-5p(SUV39H1)、hsa-mi R-152(MAML1)、hsa-mi R-1(FOXD3)、hsa-mi R-146b-5p(MEF2C)、hsa-mi R-152(DOT1L)、hsa-mi R-493-5p(FBXW7)、hsa-mi R-876-5p(PIK3R1)、hsa-mi R-183-3p(MDM2)、hsa-mi R-382-3p(CD80)、hsa-mi R-181b-5p(SIRT1)、hsa-mi R-874-5p(MTOR)、hsa-mi R-876-5p(GPRC6A)等micro RNAs-靶基因参与涉及Erb B信号通路、细胞凋亡信号通路、B细胞受体信号通路、磷脂酰肌醇代谢信号通路等,在PTC疾病发生发展中可能起着重要作用。从分子水平上分析甲状腺乳头状癌相关致病基因及micro RNAs,为揭示PTC发病机制、药物研发及临床诊断治疗提供新的思路和依据。
[Abstract]:The purpose of this study was to obtain papillary thyroid carcinoma (PTC) by using the NCBI-based gene expression database of the National Center for Biotechnology Information (NCBI) of the United States (NCBI). Papillary thyroid carcinoma. PTC)m RNA expression microarray was used in 7 PTC samples and 7 normal controls. The login number was GSM85-215GSM 85228) and miro RNA high-throughput sequencing data were obtained from 3 PTC samples and 3 normal controls. GSM1388420 GSM138-8425. Through Qlucore Omics Explorer, QOEO 3.2i DAVID 6.7. STING 9.1. Analysis of differential genes and micro in PTC by mi Records et al. A total of 497 thyroid papillary carcinoma-associated genes and 86 related micro RNs were screened by comprehensive bioinformatics analysis by RNA. Among them, 257 genes were up-regulated and 240 were down-regulated. In micro RNAs, 47 were up-regulated and 39 down-regulated. Bioinformatics analysis revealed that ERBB3, TNNT1 and MYL1 were detected. POSTN gene and hsa-mi R-183-5pPERBB4, hsa-mi R-139-5pPU SUV39H1). Hsa-mi R-152 (MAML1), hsa-mi R-1 (FOXD3), HSA-mi R-146b-5pOMEF2C). Hsa-mi R-152DOT1L (HSA-mi R-493-5pN FBXW7 / HSA-mi R-876-5pN PIK3R1). Hsa-mi R-183-3pM2M2HSA-mi R-382-3pTCD80 / hsa-mi R-181b-5pSRT1). Hsa-mi R-874-5pMTOR). Micro RNAs-target genes such as hsa-mi R-876-5pGPRC6A) are involved in Erb B signaling pathway and apoptosis signaling pathway. B cell receptor signaling pathway, phosphatidylinositol metabolic signaling pathway and so on. It may play an important role in the occurrence and development of PTC disease. The molecular analysis of thyroid papillary carcinoma-associated genes and micro RNASs is intended to reveal the pathogenesis of PTC. Research and development of drugs and clinical diagnosis and treatment provide new ideas and basis.
【作者单位】：北京理工大学医院;北京理工大学;
【基金】：国家自然科学基金(项目号:11372042)资助
【分类号】：R736.1
【正文快照】： *通讯作者,lhy_science@163.comScreening and Bioinformatics Analysis of Thyroid Papillary CarcinomaRelated Genes and Micro RNAsHan Youxia1Fan Guiping1Ji Baohua1Liu Haiyan1*1 Beijing Institute of Technology Hospital,Beijing,100081;2 Beijing Institute of Tec

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