RNPC1与p53基因在肺腺癌组织中的表达及其临床意义

发布时间：2018-02-04 13:49

本文关键词： 肺肿瘤肺腺癌 RNPC1 RBM38 p53　出处：《新疆医科大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:观察肺腺癌组织中RNPC1、p53基因表达及其与肺腺癌临床特征之间的关系,探讨其在肺腺癌的发生发展中的意义。方法:采用RT-PCR和Western blot法检测50例肺腺癌组织及对应癌旁组织中RNPC1、p53 mRNA及蛋白水平的相对表达量,分析其与肺腺癌临床病理特征之间的关系,及其两者之间的关系。结果:肺腺癌RNPC1 mRNA及蛋白相对表达量(0.357±0.170、0.294±0.149)均高于癌旁组织(0.271±0.128、0.206±0.099),肺腺癌组织p53 mRNA及蛋白(0.457±0.208、0.671±0.200)相对表达量均高于癌旁组织(0.308±0.167、0.332±0.071),差异有统计学意义(P0.01)。RNPC1表达与肺腺癌患者TNM分期、浸润深度有关(P0.05)。p53表达与患者TNM分期有关(P0.05)。实验组RNPC1与p53蛋白表达相关(r=-0.626,P0.01)。结论:肺腺癌组织RNPC1、p53 mRNA及蛋白表达高于癌旁组织,两者均与患者部分病理参数关系密切。随着RNPC1基因表达的增加p53蛋白随之减少,RNPC1可能通过抑制p53的翻译从而促进肺癌的发生发展。RNPC1可能成为肺腺癌分子靶向治疗的靶点。
[Abstract]:Objective: to investigate the relationship between the expression of RNPC1 and p53 gene and the clinical features of lung adenocarcinoma. Methods: RT-PCR and Western blot were used to detect RNPC1 in 50 cases of lung adenocarcinoma and its adjacent tissues. The relative expression of p53 mRNA and protein, and the relationship between p53 mRNA and clinicopathological features of lung adenocarcinoma were analyzed. Results: the relative expression of RNPC1 mRNA and protein in lung adenocarcinoma was 0.357 卤0.170. 0.294 卤0.149) was higher than that of paracancerous tissue (0.271 卤0.128 卤0.206 卤0.099). The relative expressions of p53 mRNA and protein in lung adenocarcinoma were higher than those in paracancerous tissues (0.457 卤0.208 卤0.671 卤0.200). The difference was statistically significant between the expression of P0.01P. RNPC1 and the TNM stage of lung adenocarcinoma. The depth of invasion was related to the expression of P0.05, p53 and the TNM stage of the patients. The expression of RNPC1 and p53 protein was correlated with r-0.626 in the experimental group. Conclusion: the expression of p53 mRNA and protein in lung adenocarcinoma tissue is higher than that in paracancerous tissue. The expression of p53 protein decreased with the increase of RNPC1 gene expression. RNPC1 may promote the development of lung cancer by inhibiting the translation of p53. RNPC1 may be the target of molecular targeted therapy for lung adenocarcinoma.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R734.2

【参考文献】