不同地理种群白纹伊蚊线粒体基因COI的遗传多样性分析

发布时间：2018-02-04 23:38

本文关键词： 白纹伊蚊地理隔离瓶颈效应蚊媒病　出处：《中国人兽共患病学报》2017年04期 　论文类型：期刊论文

【摘要】：目的探讨白纹伊蚊不同地理种群间的进化关系和遗传分化情况,为白纹伊蚊防制和蚊媒病防控提供基础资料。方法在广泛采集样品的基础上,通过PCR扩增、测序获取线粒体基因COI片段,并从GenBank下载了部分序列,比对、剪切后的598bp用于后续分析。结果系统发育分析的结果表明所有白纹伊蚊的COI序列聚成一支,没有明显的遗传差异;60条COI序列分属于19个单倍型,其中4个为共享单倍型;单倍型多样性(Hd)为0.737,核苷酸多样性(π)为0.20%;海南的白纹伊蚊种群与绝大部分地理种群间出现了明显的分化(P0.05);H1和H6形成了2个辐射中心,是较为原始的单倍型。结论我国的白纹伊蚊种群正处于扩张的趋势,海南的白纹伊蚊种群与其它地理种群间出现了明显分化。
[Abstract]:Objective to study the evolutionary relationship and genetic differentiation of Aedes albopictus among different geographical populations, and to provide basic data for control of Aedes albopictus and mosquito vector disease. The mitochondrial gene COI fragment was obtained by sequencing, and some sequences were downloaded from GenBank and compared with each other. Results the results of phylogenetic analysis showed that all the COI sequences of Aedes albopictus were clustered into one branch, and there was no obvious genetic difference. 60 COI sequences belong to 19 haplotypes, 4 of which are shared haplotypes. Haplotype diversity (Hd) was 0.737, nucleotide diversity (蟺) was 0.20; The population of Aedes albopictus in Hainan was significantly differentiated from most geographic populations (P0.05). Conclusion the population of Aedes albopictus in China is in the trend of expansion, and the population of Aedes albopictus in Hainan is obviously differentiated from other geographical populations.
【作者单位】：泰山医学院新发传染病溯源及防控协同创新中心;泰山医学院基础医学院;泰安市疾病预防控制中心;
【基金】：国家自然科学基金(Nos.81401693&81572028) 大学生创新创业训练计划(No.201510439010)联合资助~~
【分类号】：R384.1
【正文快照】： 白纹伊蚊(Aedes　albopictus)又称“亚洲虎蚊”,ddH2O,21μL。PCR反应条件为:95℃预变性隶属于双翅目,蚊科,伊蚊属,是中国常见蚊种之一。5min;95℃变性30s,45℃复性50s,72℃延伸60s,白纹伊蚊是登革热、黄热病、基孔肯雅热、西尼罗热共35个循环;最后72℃延伸10min。等多种病毒

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