实时荧光定量RT-PCR检测赖草LRC1基因表达研究

发布时间：2018-02-04 22:51

  本文关键词： 赖草 根茎 分蘖基因 实时荧光定量RT-PCR　出处：《中国草地学报》2017年05期 　论文类型：期刊论文

【摘要】：通过亚克隆技术获得赖草LRC1基因片段重组质粒,以其梯度稀释样品为标准,建立了实时荧光定量PCR技术检测LRC1基因绝对定量表达方法。进一步运用该技术和相对定量法对赖草初花期LRC1基因表达特性进行分析发现,该基因在根茎尖、根茎节间、根茎节、地上茎中的拷贝数分别为477.79、162.65、1368.00、53.86个拷贝/mg(组织鲜重);相对定量法检测结果显示,LRC1基因在赖草根茎节间中的表达量约为其在根茎节中表达量的10%,穗、叶片和茎中表达量则为其在根茎节中的1%、2%和1%。表明该基因在赖草根茎中的表达量显著高于地上组织,特别是其在根茎芽产生的根茎节内的表达量显著高于其他组织。
[Abstract]:The recombinant plasmid of LRC1 gene fragment of Leymus chinensis was obtained by subcloning technique, and its gradient dilution was used as the standard. A real-time fluorescence quantitative PCR method was established to detect the absolute quantitative expression of LRC1 gene, and the characteristics of LRC1 gene expression in the early flowering stage of Rhizoma Raigrass were analyzed by using this technique and the relative quantitative method. The number of copies of the gene in rhizome tip, rhizome Internode, rhizome node and upper stem were 477.79 ~ 162.65.1368.00,53.86 copies / mg (fresh tissue weight), respectively. The results of relative quantitative analysis showed that the expression of LRC1 gene in rhizome Internode of Leymus chinensis was about 10% of that in rhizome node, and that in ear, leaf and stem was 1% of that in rhizome node. 2% and 1.The results showed that the expression of the gene in the rhizome of Leymus chinensis was significantly higher than that in the aboveground tissue, especially in the rhizome node produced by the rhizome bud.
【作者单位】： 济南大学生物科学与技术学院;山东无棣县农业局;
【基金】：国家自然科学基金项目(30700561,31372356)
【分类号】：S543.9
【正文快照】： 赖草属(Leymus Hochst.)是禾本科早熟禾亚科小麦族中的多年生植物类群[1]。全世界约有30个种19个亚种,分布于北半球温寒地带、亚洲中部,少数分布于欧洲和北美。我国原产约有27个种、3个亚种和3个变种,主要分布于西北、华北、东北及西南地区。赖草属植物生境多样,具有广泛的适，

本文编号：1491377