人参皂苷生物合成基因组织表达特性的研究

发布时间：2018-02-08 09:44

本文关键词： 人参人参皂苷生物合成基因组织表达　出处：《中国中药杂志》2017年13期 　论文类型：期刊论文

【摘要】：采用生物信息学及实时荧光定量方法对10个人参皂苷生物合成关键酶基因的组织表达特性进行研究。运用转录组热图聚类方法分析了四年生吉林人参14个不同部位人参皂苷生物合成基因的表达;运用实时荧光定量PCR的方法测定人参组培苗、不定根中人参皂苷生物合成基因的表达;运用Pearson相关对这10个人参皂苷生物合成关键酶基因表达特性进行分析。结果表明,β-AS,CYP716A52v2在吉林人参及组培苗的根中表达高,与Ro分布相一致;与达玛烷型人参皂苷合成相关的CYP716A47,CYP716A53v2表达呈显著正相关,从分子水平证明了人参皂苷化学成分分布的差异主要由转运引起。
[Abstract]:The tissue expression characteristics of 10 key enzyme genes of ginsenoside biosynthesis were studied by bioinformatics and real-time fluorescence quantitative method. Fourteen different parts of Jilin Ginseng were analyzed by transcriptome heat map clustering method. Expression of ginsenoside biosynthesis gene; The expression of ginsenoside biosynthesis gene in adventitious roots and tissue culture plantlets of Panax ginseng was determined by real-time fluorescence quantitative PCR. The expression characteristics of the key enzymes of ginsenoside biosynthesis were analyzed by Pearson correlation. The results showed that 尾 -AS-CYP716A52v2 was highly expressed in the roots of Jilin ginseng and its tissue culture seedlings, which was consistent with the distribution of Ro. There was a significant positive correlation between CYP716A47 and CYP716A53v2 expression related to the synthesis of dharmane type ginsenoside. It was proved that the difference of chemical composition distribution of ginsenoside was mainly caused by transport at molecular level.
【作者单位】：中国中医科学院中药资源中心道地药材国家重点实验室培育基地;中国农业科学院特产研究所;天津大学药物科学与技术学院;
【基金】：国家自然科学基金项目(81603235) 国家杰出青年科学基金项目(81325023) 中央本级重大增减支项目(2060302) 中国中医科学院中药资源中心自主选题研究项目(ZZ2016006)
【分类号】：S567.51

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