HOG 1基因在小鼠系统性白念珠菌感染中的致病性作用

发布时间：2018-02-07 16:30

本文关键词： HOG 基因念珠菌病动物实验毒力　出处：《中国皮肤性病学杂志》2017年01期 　论文类型：期刊论文

【摘要】：目的探讨HOG 1基因在小鼠系统性白念珠菌感染中的作用。方法建立小鼠系统性白念珠菌感染动物模型,设置实验组,HOG 21组(hog1/hog1双等位基因缺陷株);对照组,CAF-2组(标准株)。进行死亡率观察并描绘生存曲线;平皿稀释法检测脾肾组织菌落形成单位(CFU)数目;PAS染色法评估肾脏组织病理学改变。结果实验组小鼠死亡率为0;对照组小鼠死亡率100%;感染后第1~3天,实验组小鼠脾肾带菌量CFU值明显低于对照组(P0.05),差异有统计学意义;感染后第3天和第5天,实验组小鼠肾脏组织病理检查见小脓肿和微小脓肿,偶见菌丝和孢子;对照组小鼠见大量的菌丝和孢子在肾髓质肾乳头交织成团,伴有大量的中性粒细胞浸润。结论 HOG 1基因在小鼠系统性白念珠菌感染的动物模型中发挥一定的致病性作用。
[Abstract]:Objective to investigate the role of HOG 1 gene in systemic Candida albicans infection in mice. Two allelic deficiency strains of hog 1 / hog 1 were set up in the experimental group and CAF-2 group (standard strain) were used to observe the mortality and draw the survival curve. The number of colony forming units (CFU) in spleen and kidney was detected by plate dilution method and pas staining method was used to evaluate the pathological changes of kidney. Results the mortality rate of experimental group was 0; that of control group was 100; the 1st day after infection was 3 days. The CFU value of spleen and kidney carrying bacteria in the experimental group was significantly lower than that in the control group (P 0.05), and on the 3rd and 5th day after infection, the small abscess and microabscess were found in the kidney tissue of the experimental group, and the hyphae and spores were occasionally found in the experimental group. In the control group, a large number of mycelium and spores were found in the renal medullary papilla, accompanied by a large number of neutrophil infiltration. Conclusion HOG 1 gene plays a certain pathogenicity in the animal model of systemic Candida albicans infection in mice.
【作者单位】：厦门大学附属第一医院皮肤科暨福建医科大学厦门市第一医院教学医院皮肤科;福建医科大学附属第一医院皮肤科;
【分类号】：R756

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