玉米热激转录因子基因ZmHsf06表达提高拟南芥耐盐性

发布时间：2018-02-08 22:58

本文关键词： 玉米 ZmHsf 热激转录因子盐胁迫基因表达　出处：《河北农业大学学报》2016年02期 　论文类型：期刊论文

【摘要】：在对从玉米幼叶中获得热激转录因子基因ZmHsf06生物学特性及转基因拟南芥植株耐热性和抗旱性评价的基础上,从表型和生理水平对转ZmHsf06基因植株的耐盐性进行了鉴定。表型观察结果显示,盐胁迫下转ZmHsf06拟南芥植株根系和地上部长势明显强于野生型;生理指标分析发现,盐胁迫下转基因植株能保持相对较高的叶片SOD、POD、CAT活性和叶绿素含量,以及相对较低的相对电导率、丙二醛含量,且叶片渗透势升高明显。结果表明,ZmHsf06不仅能提高植株耐热性和抗旱性,在一定程度上对提高植株的耐盐性也发挥重要作用,是玉米热激转录因子家族主要成员之一。
[Abstract]:The biological characteristics of heat shock transcription factor gene (ZmHsf06) obtained from young leaves of maize and the heat tolerance and drought resistance of transgenic Arabidopsis thaliana plants were evaluated. The salt tolerance of transgenic ZmHsf06 transgenic plants was identified by phenotypic and physiological levels. Phenotypic observation showed that the root and shoot potential of transgenic ZmHsf06 Arabidopsis plants under salt stress was significantly stronger than that of wild type. Under salt stress, transgenic plants could maintain relatively high activity of POD POD cat and chlorophyll content in leaves, as well as relatively low relative electrical conductivity and malondialdehyde content. The results showed that ZmHsf06 could not only improve the heat resistance and drought resistance of plants, but also play an important role in improving the salt tolerance of plants to some extent. ZmHsf06 was one of the main members of heat shock transcription factor family in maize.
【作者单位】：河北省农林科学院遗传生理研究所/河北省植物转基因中心重点实验室;石家庄市农林科学研究院;
【基金】：河北省应用基础研究重点研究项目(12965517D)
【分类号】：S513

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