C3和DAF基因多态与胃癌遗传易感性关系的研究

发布时间：2018-02-09 11:21

本文关键词： DAF 标签SNP 单核苷酸多态胃癌补体系统　出处：《华北理工大学》2016年硕士论文　论文类型：学位论文

【摘要】：目的补体系统通过参与机体对抗外来物质的炎症反应,改变机体微环境而影响肿瘤发生。本研究旨在探讨补体关键基因C3和DAF标签遗传变异与胃癌发病风险的关系,为胃癌的早期发现,易感人群筛查提供理论依据。方法研究对象来自2008年2月到2013年12月期间在华北理工大学附属唐山市工人医院和华北理工大学附属唐山市人民医院就诊的500例胃癌患者,以及500例同期健康查体个体。利用Hapmap公共数据库提供的数据,运用Haploview4.2软件选取补体C3和DAF基因的标签单核苷酸多态(tag SNP)。使用高通量的Sequenom Mass Array方法对所有的标签SNP在1000例研究对象中进行基因分型。用卡方检验分析病例组和对照组年龄、性别和吸烟状况等基本人口学资料之间的差异。用非条件Logistic回归模型计算各遗传变异与胃癌发病风险的OR值及其95%CI值。结果使用Haplo View软件我们获得了位于补体C3的12个tag SNP(rs220199,rs2230205,rs2241393,rs2250656,rs2277984,rs2287846,rs3745568,rs432001,rs11569523,rs379527,rs2230204,rs433594)和位于DAF基因的2个tag SNP(rs10746463,rs2564978)用于进一步研究。DAF rs10746463 GG、GA和AA三种基因型在病例组和对照组中分别占27.0%、52.2%、20.8%和34.0%、48.4%、17.6%。Logistic回归分析结果显示,DAF rs10746463多态与胃癌发病风险相关。与携带rs10746463 GG基因型的个体相比,AA基因型的携带者具有较高的胃癌发病风险,其OR值(95%CI)值为1.46(1.01-2.10)。与携带DAF rs10746463的非吸烟者相比,携带至少一个A等位基因的吸烟者具有较高的胃癌发病风险(OR=1.64;95%CI=1.06-2.54);而携带至少一个A等位基因的非吸烟者发生胃癌的风险没有改变(OR=1.37;95%CI=0.97-1.94)。我们的数据并没有发现DAF rs2564978基因多态以及C3 tag SNP与胃癌遗传易感性之间的关系。结论在中国北方人群中,DAF rs10746463遗传变异影响胃癌发病风险,而且该遗传变异和吸烟存在交互作用共同增加胃癌发病风险。
[Abstract]:Objective to investigate the relationship between the genetic variation of complement key genes C3 and DAF and the risk of gastric cancer. For early detection of gastric cancer, Methods from February 2008 to December 2013, 500 patients with gastric cancer were selected from the Tangshan Workers Hospital affiliated to North China University of Technology and the people's Hospital of Tangshan City, affiliated to North China University of Technology. Using data from the Hapmap public database, The single nucleotide polymorphism of complement C3 and DAF gene was selected by Haploview4.2 software. All tagged SNP was genotyped in 1000 subjects by high-throughput Sequenom Mass Array method. The age of case group and control group were analyzed by chi-square test. The difference between basic demographic data such as sex and smoking status. The OR value and 95 CI value of each genetic variation and gastric cancer risk were calculated by non-conditional Logistic regression model. Results using Haplo View software, we obtained the complement C3. Twelve tag SNPRs220199rs2230205rs2241393rs2277984rs228784rs3745568rs432001rs11569523rs3727rs2230204rs433594) and two tag SNPRs10746463rs2564978) were used to further study the risk of gastric cancer. Compared with individuals with rs10746463 GG genotype, carriers with AA genotype had a higher risk of gastric cancer. Its OR value was 1.46 ~ 1.01-2.100.Compared with non-smokers carrying DAF rs10746463, Smokers with at least one A allele had a higher risk of developing gastric cancer, OR1.64 / 95 CI 1.06-2.54, while non-smokers with at least one A allele had no change in the risk of developing gastric cancer, OR1.3795CI0.97-1.94. Our data did not find the DAF rs2564978 gene. The relationship between polymorphism and C 3 tag SNP and the genetic susceptibility of gastric cancer. Conclusion the genetic variation of rs10746463 in the population of North China has an effect on the risk of gastric cancer. In addition, the genetic variation and smoking have an interaction to increase the risk of gastric cancer.
【学位授予单位】：华北理工大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R735.2

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