玉米ZmWRKY45基因克隆及盐碱逆境胁迫下的表达分析

发布时间：2018-02-11 13:33

  本文关键词： 玉米 ZmWRKY基因 电子克隆 盐碱胁迫　出处：《玉米科学》2017年03期 　论文类型：期刊论文

【摘要】：通过水稻中OsWRKY45基因在NCBI中进行同源序列比对,电子克隆ZmWRKY45基因并与其他物种中的同源基因进行进化树分析。利用该基因的CDS区设计特异引物,在3叶期对吉单441进行不同浓度NaHCO_3处理,对玉米杂交种吉单441及其双亲自交系进行验证。结果表明,在吉单441及其父本中含有ZmWRKY45,在盐碱胁迫下吉单441中该基因随着盐碱浓度的升高而表达量升高,初步判断该基因可能在吉单441的耐盐碱过程中起到正向调控作用。
[Abstract]:OsWRKY45 gene in rice was sequenced in NCBI, then ZmWRKY45 gene was cloned and analyzed with the phylogenetic tree of other species. Specific primers were designed using the CDS region of this gene. Jidan 441 was treated with different concentrations of NaHCO_3 at 3 leaf stage, and the maize hybrid Jidan 441 and its parents inbred lines were tested. There was ZmWRKY45in Jidan 441 and its male parent. Under salt and alkali stress, the expression of Jidan 441 increased with the increase of saline-alkali concentration. It was preliminarily concluded that the gene might play a positive role in the saline-alkali tolerance of Jidan 441.
【作者单位】： 吉林省农业科学院;
【基金】：“十三五”国家重点研发专项“玉米分子设计育种课题”(YFD0101803)
【分类号】：S513
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本文编号：1503177