冠心病不稳定性心绞痛血瘀证患者IL-6基因甲基化研究及方法探讨

发布时间：2018-02-11 20:16

本文关键词： 冠心病不稳定性心绞痛血瘀证 DNA甲基化表观遗传学亚硫酸氢盐质谱　出处：《中国实验方剂学杂志》2017年19期 　论文类型：期刊论文

【摘要】：目的:研究冠心病不稳定性心绞痛(unstable angina,UA)患者外周血细胞中白细胞介素-6(IL-6)基因启动子区甲基化状态与中医血瘀证的关系。方法:选取6例2015年2月—2015年5月中国中医科学院广安门医院心血管科住院或门诊UA血瘀证患者作为试验组,另选取6例中国中医科学院广安门医院心内科住院或门诊UA非血瘀证患者作为对照组,采用重亚硫酸盐处理检测两组IL-6基因启动子区甲基化状态并进行组间比较,分析血瘀证与基因启动子区甲基化相关性。结果:IL-6基因转录起始位点前-1 118 bp至-826 bp序列中7个位点Cp G位点甲基化程度血瘀证组存在高于非血瘀证组的趋势,但差异尚无统计学意义。IL-6基因转录起始位点前-1 47 1 bp至-1 184 bp序列4个Cp G位点甲基化程度血瘀证组与非血瘀证组之间的差异尚不明显。结论:UA血瘀证患者IL-6基因启动子甲基化水平存在高于非血瘀证组的趋势,一定程度反映了UA血瘀证本质。
[Abstract]:Objective: to study the relationship between methylation status of interleukin-6 (IL-6) gene promoter region and blood stasis syndrome in 6 patients with unstable angina pectoris (UAA) from February 2015 to May 2015. The patients with UA blood stasis syndrome in cardiovascular department or outpatient department of Guanganmen Hospital of Medical College were used as trial group. In addition, 6 inpatients with non-blood stasis syndrome of UA in Department of Cardiology, Guanganmen Hospital, Chinese Academy of traditional Chinese Medicine, were selected as control group. The methylation status of promoter region of IL-6 gene in two groups was detected by heavy sulfite treatment and compared between the two groups. Results the methylation degree of 7 CpG loci in the first 1 118 BP to -826 BP sequence of the 1: IL-6 gene transcriptional initiation site was higher than that in the non blood stasis syndrome group. But there is no significant difference between the four CpG methylation degree blood stasis syndrome group and the non-blood stasis syndrome group. Conclusion the IL-6 of patients with blood stasis syndrome of blood stasis syndrome is not obvious. The methylation level of gene promoter was higher than that of non-blood stasis group. To some extent, it reflects the essence of UA blood stasis syndrome.
【作者单位】：中国中医科学院广安门医院;北京中医药大学;首都儿科研究所;
【基金】：国家自然科学基金项目(81473561;81673847) 国家“重大新药创制”科技重大专项(2013ZX09301307)
【分类号】：R259

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