新疆油鸡H9N2亚型禽流感病毒PB1基因的克隆与序列分析

发布时间：2018-02-11 21:39

本文关键词： 流感病毒油鸡聚合酶PB基因克隆序列分析　出处：《西南农业学报》2017年01期 　论文类型：期刊论文

【摘要】：为了阐明新疆油鸡H9亚型流感病毒PB1基因的分子特征与进化趋势,本研究采用RT-PCR技术对新疆油鸡分离株A/Chicken/Xinjiang Baicheng/1/2014(H9 N2)的PB1基因进行了克隆、测序及分子进化分析,将PB1基因的核苷酸序列和对应的氨基酸序列与Genbank中已经公布的参考序列进行同源性比较。结果表明,新疆油鸡分离株的PB1基因由2274个碱基组成,编码757个氨基酸,与AIV A/chicken/Henan/89/2013(H7N9)的PB1基因处于同一分支,其核苷酸和氨基酸水平的同源性依次为99.2%和99.4%,新疆油鸡分离株的PB1基因不属于欧亚分支G1、A/CK/BJ/94和Y439的任何谱系。
[Abstract]:In order to elucidate the molecular characteristics and evolutionary trend of PB1 gene of Xinjiang oil chicken H9 subtype influenza virus, the PB1 gene of Xinjiang oil chicken isolate A / Chicken / Xinjiang Baicheng/1/2014(H9 N2 was cloned, sequenced and analyzed by RT-PCR. The nucleotide sequence and corresponding amino acid sequence of PB1 gene were compared with the reference sequence of Genbank. The results showed that the PB1 gene of Xinjiang oil chicken isolate was composed of 2274 bases and encoded 757 amino acids. The nucleotide and amino acid homology of PB1 gene of AIV A / chicken / Henan / 89 / 2013 H7N9) was 99.2% and 99.4, respectively. The PB1 gene of Xinjiang oil chicken isolate did not belong to any lineage of Eurasian branch G1Ar / CK / BJ94 and Y439.
【作者单位】：塔里木大学;新疆生产建设兵团塔里木畜牧科技重点实验室;新疆库车县动物疾病预防控制中心;阿克苏地区动物疫病控制诊断中心;新疆阿瓦提县畜禽改良站;
【基金】：国家自然科学基金(31160513)
【分类号】：S852.65

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