ADAMTS1基因多态性与冠心病的相关性研究

发布时间：2018-02-13 10:52

  本文关键词： 含人类Ⅰ型血小板结合蛋白基序的解聚蛋白样金属蛋白酶- 单核苷酸多态性 冠心病　出处：《重庆医科大学学报》2016年12期 　论文类型：期刊论文

【摘要】：目的:探讨ADAMTS1基因SNPrs428785和SNPrs2738两个位点与冠心病(coronary heart disease,CHD)的相关性。方法:采用聚合酶链反应结合单链构象多态性和聚合酶链反应结合限制性片段长度多态性,再结合DNA测序技术对315例CHD患者和355例对照人群进行SNPrs428785和SNPrs2738检测,2组间计数资料比较采用卡方检验,计量资料比较采用独立样本t检验和Mann-Whitney U检验分析。结果:SNPrs428785在CHD组和对照组中基因型频率为CC(50.2%vs 38.9%)、CG(40.3%vs.47.9%)和GG(9.5%vs.13.2%),差异有统计学意义(χ~2=8.974,P=0.011),2组间G等位基因频率分别为29.7%和37.2%,差异有统计学意义(χ~2=8.410,P=0.004),经二元logistic回归分析后,提示rs428785的基因型与冠心病的发生相关(OR=1.528,95%CI=1.086~2.150,P=0.015)。SNPrs2738在CHD组和对照组中的基因型频率为AA(74.3%vs.78.9%)、AC(24.1%vs.18.9%)和CC(1.6%vs.2.3%),差异无统计学意义(χ~2=2.998,P=0.223),A等位基因频率分别为86.3%和88.3%,差异无统计学意义(χ~2=1.164,P=0.281)。结论:ADAMTS1基因SNPrs428785多态性与冠心病发生相关,且G等位基因可能是冠心病的遗传易感基因,而SNPrs2738多态性与冠心病发生无相关性。
[Abstract]:Objective: to investigate the relationship between SNPrs428785 and SNPrs2738 of ADAMTS1 gene and coronary heart disease (CHD). Methods: polymerase chain reaction (PCR) combined with single strand conformation polymorphism (SSCP) and polymerase chain reaction (PCR) combined with restriction fragment length polymorphism (RFLP) were used. Combined with DNA sequencing technique, the counting data of 315 CHD patients and 355 controls were compared between SNPrs428785 and SNPrs2738 detection group by chi-square test. The quantitative data were analyzed by independent sample t test and Mann-Whitney U test. Results the frequency of G allele in CHD group and control group was CC(50.2%vs 38.9% and CC(50.2%vs 38.9%, respectively. The difference was statistically significant (蠂 2 8.974P0. 011) and G allele frequencies were 29.7% and 37.2%, respectively, and the difference was statistically significant (蠂 2 8.974P0. 011), the difference was significant (蠂 2 8.974 P0. 01111), the frequency of G allele was 29.7% and 37. 2% respectively, and the difference was significant (蠂 2 8.97 4 P 0. 011 1), the difference was significant (蠂 2 8. 97 4 P 0. 011). There was statistical significance (蠂 ~ 2 ~ 2 ~ (8.410) P ~ (0.004)). After binary logistic regression analysis, The results suggest that the genotype of rs428785 is related to the occurrence of coronary heart disease. The frequency of genotype of rs428785 in CHD group and control group is AA74.3vs.78.9s. There is no significant difference between CHD group and control group. Conclusion there is no significant difference in allele frequency of ADAMTS1 gene between CHD group and control group. Conclusion there is no significant difference in allele frequencies of ADAMTS1 gene between CHD group and control group (蠂 ~ 22.998A = 86.3% and 88.323A, respectively). Conclusion there is no significant difference in allele frequencies of ADAMTS1 gene between CHD group and control group (P < 0.01). Conclusion there is no significant difference in allele frequencies of ADAMTS1 gene between CHD group and control group (P < 0.05). Conclusion there is no significant difference in allele frequencies of ADAMTS1 gene between CHD group and control group. SNPrs428785 polymorphism is associated with coronary heart disease. G allele may be a genetic susceptibility gene to coronary heart disease, but SNPrs2738 polymorphism is not associated with coronary heart disease.
【作者单位】： 重庆医科大学附属第一医院临床研究中心;
【分类号】：R541.4
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本文编号：1507991