妇科患者表皮葡萄球菌耐药及相关基因分析

发布时间：2018-02-13 13:42

本文关键词： 妇科患者表皮葡萄球菌耐药性黏附基因 PCR检测　出处：《中国病原生物学杂志》2017年12期 　论文类型：期刊论文

【摘要】：目的分析妇科患者表皮葡萄球菌耐药及相关黏附基因分布情况,为临床感染疾病的防治提供指导。方法收集本院妇科患者不同标本,培养分离后经全自动细菌鉴定仪鉴定表皮葡萄球菌,用K-B法分析菌株耐药性,经PCR扩增后进行凝胶电泳检测黏附基因。结果 354株表皮葡萄球菌临床分离株中,来源于血液标本216株(占61.02%),阴道分泌物42株(占11.86%),手术切口分泌物32株(占9.04%),导管引流物25株(占7.06%),脓液21株(占5.93%),尿液18株(占5.08%)。354株表皮葡萄球菌对氯霉素、青霉素、红霉素、环丙沙星、莫西沙星、左氧氟沙星、庆大霉素和呋喃妥因耐药分别为237、216、191、124、113、92、67和39株,耐药率分别为66.95%、61.02%、53.95%、35.03%、31.92%、25.99%、18.93%和11.02%。表皮葡萄球菌对万古霉素和利奈唑胺仍然敏感。354株分离菌中,38株检出mecA基因,检出率为10.73%,15株携带icaA基因,检出率为4.24%,10株携带icaD基因,检出率为2.82%。结论表皮葡萄球菌主要来源于血液标本,临床抗感染治疗可以优先使用万古霉素和利奈唑胺。表皮葡萄球菌主要携带的黏附基因类型为mecA基因,其次为icaA基因和icaD基因,黏附基因的存在可能影响菌株的致病性及耐药性。
[Abstract]:Objective to analyze the distribution of drug resistance and associated adhesion genes of staphylococcus epidermidis in gynecological patients so as to provide guidance for the prevention and treatment of clinical infectious diseases. After culture, Staphylococcus epidermidis was identified by automatic bacterial identification instrument. Drug resistance of Staphylococcus epidermidis was analyzed by K-B method. The adhesion gene was detected by gel electrophoresis after PCR amplification. Results among 354 clinical isolates of Staphylococcus epidermidis, 216 blood samples (61.02%), 42 vaginal secretions (11.86%), 32 operative incision secretions (9.04%), 25 catheter drainage (7.06%), 21 pus (5.933%), 18 urine (5.08% 5.354 strains of Staphylococcus epidermidis, penicillin), The resistance of erythromycin, ciprofloxacin, moxifloxacin, levofloxacin, gentamicin and furantoin were 237, 216, 191, 124, 113, 922, 67 and 39, respectively. The resistance rates of Staphylococcus epidermidis to vancomycin and linazolamine were 18.93% and 11.02%, respectively. 38 strains of Staphylococcus epidermidis were still sensitive to vancomycin and linazolamine. The detection rate was 10.7315 strains carrying icaA gene, and the detection rate was 4.2424%. Conclusion Staphylococcus epidermidis is mainly derived from blood samples. Vancomycin and linazolamide can be used preferentially in clinical anti-infection therapy. The main adhesion gene type of Staphylococcus epidermidis is mecA gene. The presence of adhesion gene may affect the pathogenicity and drug resistance of the strain.
【作者单位】：河南省直第三人民医院;郑州大学第三附属医院;
【分类号】：R440;R711

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