应用直接PCR技术快速筛查转基因玉米方法研究

发布时间：2018-02-14 09:06

本文关键词： 转基因玉米直接PCR 快速筛查　出处：《玉米科学》2017年01期 　论文类型：期刊论文

【摘要】：以转CP4-epsps基因玉米为研究对象,用玉米内标准基因z SSIIb及3种常见转基因成分(Ca MV35S启动子、NOS终止子、CP4-epsps基因)为检测靶标,建立基于直接PCR技术的玉米转基因成分筛查方法。此方法不用提取纯化DNA,只需取直径约为0.5 mm的叶片加到PCR反应体系中进行扩增,与常规PCR方法相比,在保证结果准确性的同时,大大缩短检测时间,提高工作效率。此方法具有操作简便、结果可靠等优点,适用于玉米叶片中转基因成分的快速筛查。
[Abstract]:The CP4-epsps transgenic maize as the research object, the use of corn in standard Z gene SSIIb and 3 kinds of genetically modified (Ca MV35S promoter, NOS terminator, CP4-epsps gene) for target detection, the establishment of genetically modified maize screening method based on PCR technology. This method not only for extraction and purification of DNA were amplified in leaves PCR added to the reaction system and a diameter of about 0.5 mm, compared with the conventional PCR method, with high accuracy and shorten detection time, improve the work efficiency. This method has the advantages of simple operation, reliable advantages, suitable for rapid screening of genetically modified maize leaves.

【作者单位】：吉林省农业科学院农业质量标准与检测技术研究所;
【基金】：吉林省农业科技创新工程项目(2013)
【分类号】：S513

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