西瓜噬酸菌Aac-5AAA ATPases基因moxR和ruvB功能研究

发布时间：2018-02-15 22:27

  本文关键词： 西瓜噬酸菌 AAA ATPases moxR ruvB　出处：《植物病理学报》2016年06期 　论文类型：期刊论文

【摘要】：AAA ATPases(ATPase associated with diverse cellular activities)是一种与细胞活性相关的酶,广泛存在于所有生物中,同时具有蛋白酶和分子伴侣活性。为了研究AAA ATPases相关基因缺失对西瓜噬酸菌(Acidovorax citrulli)的影响,本研究通过同源重组双交换的方法构建西瓜噬酸菌moxR基因以及ruv B基因全缺失突变菌株及互补菌株,测定了突变菌株的致病性、烟草过敏反应、运动性、生长能力、生物膜形成等;并利用实时荧光定量PCR方法测定了AAA ATPases与Ⅲ型分泌系统相关基因(hrc N和hrp E)、毒性蛋白相关基因(trb C和vir B)、鞭毛相关基因(fliR和fli M)、菌毛相关基因(pin C-1和pin C-2)以及AAA ATPases亚家族Ruv B相关基因(ruv A和ruv C)的关系。结果表明,moxR基因以及ruv B基因全缺失突变菌株的致病力降低、运动性减弱、生长能力下降、生物膜形成能力增强、烟草过敏性反应减弱、突变菌株中重要的致病基因hrc N和hrp E表达量显著下调,表明AAA ATPases相关基因moxR、ruv B在西瓜噬酸菌的致病力上发挥重要作用。
[Abstract]:AAA ATPases(ATPase associated with diverse cellular actives is a cell-activity related enzyme that is widely present in all organisms and has protease and molecular chaperone activity. In order to study the effect of AAA ATPases related gene deletion on Acidovorax citrullii, In this study, moxR gene and ruv B gene deletion mutant strains and complementary strains were constructed by homologous recombination and double exchange method. The pathogenicity, tobacco allergic reaction, exercise ability and growth ability of mutant strains were determined. Biofilm formation; AAA ATPases genes associated with type 鈪，

本文编号：1514023