GLI3基因表达与新疆维吾尔族先天性马蹄内翻足发病的关系

发布时间：2018-02-20 04:11

  本文关键词： 先天性马蹄内翻足 GLI基因 维吾尔族 汉族 发病关系 新疆 儿童　出处：《山东医药》2016年42期 　论文类型：期刊论文

【摘要】：目的探讨GLI3基因表达与新疆维吾尔族先天性马蹄内翻足(ICTEV)发病之间的关系。方法选择新疆地区84例ICTEV患儿(维吾尔族51例,汉族33例)和39例足部创伤正常儿童(维吾尔族25例,汉族14例),分别采用实时荧光定量PCR、Western blot法检测足部肌肉组织中的GLI3 mRNA及蛋白。结果观察组足部肌肉组织中GLI3 mRNA及蛋白相对表达量分别为0.61±0.02、68.72±3.05,对照组分别为0.07±0.01、22.38±1.74,P均0.01;观察组维吾尔族患儿足部肌肉组织中GLI3 mRNA及蛋白相对表达量分别为0.73±0.01、83.01±4.42,汉族患儿分别为0.40±0.02、56.85±3.50,P均0.05;对照组维吾尔族和汉族患儿足部肌肉组织中GLI3mRNA及蛋白相对表达量比较,P均0.05。结论 GLI3基因表达与新疆维吾尔族ICTEV的发病有一定关系,维吾尔族可能更易受GLI3基因表达水平的影响而导致ICTEV的发生。
[Abstract]:Objective to investigate the relationship between the expression of GLI3 gene and the incidence of congenital equinovarus equinovarus in Xinjiang Uygur. Methods 84 cases of ICTEV in Xinjiang (51 cases of Uygur) were selected. There were 33 cases of Han nationality) and 39 cases of normal children with foot trauma (25 cases of Uygur nationality). GLI3 mRNA and protein in muscle tissue of foot were detected by real-time fluorescence quantitative PCR Western blot. Results the relative expression of GLI3 mRNA and protein in the muscle of foot in the observation group was 0.61 卤0.02, 68.72 卤3.05, and that in the control group was 0.07 卤0.01, 22.38 卤1.74, respectively (P < 0.01). The relative expression of GLI3mRNA and protein in the muscle tissue of Uygur and Han nationality were 0.73 卤0.01 卤83.01 卤4.42 and 0.40 卤0.02 卤56.85 卤3.50 P respectively, while the relative expression of GLI3mRNA and protein in the muscle tissue of the Uygur and Han nationality in the control group were compared (P. Conclusion the expression of GLI3 gene is related to the pathogenesis of ICTEV in Xinjiang Uygur nationality. Uygur may be more susceptible to the GLI3 gene expression level and lead to the occurrence of ICTEV.
【作者单位】： 新疆医科大学附属中医医院;
【基金】：国家自然科学基金资助项目(81160215)
【分类号】：R726.8
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本文编号：1518733