慢性鼻-鼻窦炎患者鼻分泌物中金黄色葡萄球菌毒力基因的分析

发布时间：2018-02-21 04:55

本文关键词： 鼻窦炎金黄色葡萄球菌毒力基因　出处：《临床耳鼻咽喉头颈外科杂志》2017年18期 　论文类型：期刊论文

【摘要】：目的:比较慢性鼻-鼻窦炎伴鼻息肉(CRSwNP)及慢性鼻-鼻窦炎不伴鼻息肉(CRSsNP)患者鼻腔金黄色葡萄球菌携带毒力基因的差异,探索不同毒力因子与鼻窦炎、鼻息肉发病之间的关系。方法:以71例CRS患者为实验组(CRSwNP患者33例,CRSsNP患者38例),同期103例非CRS者为对照组。采用PCR方法对从鼻腔内分泌物中分离鉴定的41株金葡菌所携带的17种毒力基因进行检测。结果:①实验组seb检出率明显高于对照组(χ~2=5.864,P0.05);CRSwNP组中seb检出率高于CRSsNP组(P0.05);其余5种肠毒素基因实验组与对照组比较均差异无统计学意义(P0.05);②金葡菌黏附素在实验组及对照组中均有检出,且检出率都较高,比较实验组和对照组检出率时,差异无统计学意义(P0.05);③编码溶血素的HLα、HLβ基因在实验组和对照组中检出率都较高,且这两种基因在实验组与对照组中比较均差异无统计学意义(P0.05)。结论:携带有肠毒素基因的金葡菌更易引起CRS,且可能携带肠毒素种类越多的菌株感染与鼻息肉的发病关系越大。
[Abstract]:Objective: to compare the virulence genes of Staphylococcus aureus in patients with chronic rhinosinusitis with nasal polyps (CRSwNPN) and chronic rhinosinusitis without nasal polyps (CRSsNPN), and to explore different virulence factors and sinusitis. Methods: using 71 cases of CRS as experimental group, 33 cases with CRSsNP and 103 cases without CRS as control group. 41 strains of staphylococcus aureus isolated from nasal secretion were isolated and identified by PCR method. Results the detection rate of seb in the experiment group was significantly higher than that in the control group (蠂 2 + 5.864% P 0.05%), and the detection rate of seb in the control group was higher than that in the CRSsNP group (P 0.05), but there was no significant difference between the other five enterotoxin genes groups and the control group. P0.05Staphylococcus aureus adhesion was detected in the experimental group and the control group. The detection rate was higher in the experimental group than in the control group. There was no significant difference in the detection rate between the experimental group and the control group, and the detection rate of HL 伪 hl 尾 gene encoding hemolysin 3 was higher in both the experimental group and the control group. Conclusion: Staphylococcus aureus carrying enterotoxin gene is more likely to cause CRSs, and the infection of the strains with more kinds of enterotoxin may be related to the incidence of nasal polyps.
【作者单位】：抚州市第一人民医院耳鼻喉口腔科;南昌大学第一附属医院耳鼻咽喉头颈外科;
【分类号】：R765

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