冬小麦膨胀素基因TaEXPB8、TaEXPB10的克隆及低温表达分析

发布时间：2018-02-21 04:47

  本文关键词： 冬小麦 膨胀素 低温处理 表达分析　出处：《河南农业科学》2017年02期 　论文类型：期刊论文

【摘要】：以冬小麦强抗寒品种东农冬麦1号为试验材料,从其根中克隆膨胀素基因TaEXPB8、TaEXPB10,并分析其在低温条件下的表达情况,为研究膨胀素基因与植物抗寒性之间的关系奠定基础。结果表明,在冬小麦强抗寒品种东农冬麦1号根中克隆得到膨胀素基因TaEXPB8、TaEXPB10,二者分别编码273、271个氨基酸,二者序列与Gen Bank上公布的序列同源性分别达到99.2%、99.6%。4℃、-10℃和-20℃的低温胁迫处理均可诱导TaEXPB8、TaEXPB10基因在冬小麦强抗寒品种东农冬麦1号根中表达,尤其是TaEXPB10基因,其受诱导程度高于TaEXPB8;两基因在低抗寒品种济麦22号中总体上不受诱导,甚至受到抑制。综合比较发现,4℃、-10℃和-20℃低温胁迫下,TaEXPB8、TaEXPB10基因在强抗寒品种东农冬麦1号中的表达量均明显高于低抗寒品种济麦22号。据此推测膨胀素基因TaEXPB8、TaEXPB10与冬小麦根系的低温胁迫耐受能力具有重要的相关性,有助于进一步深入了解冬小麦根系的抗寒机制。
[Abstract]:A strong cold resistant winter wheat variety Dongnong Dongmai 1 was used to clone the expandin gene TaEXPB8 and TaEXPB10 from its roots, and the expression of TaEXPB8 and TaEXPB10 at low temperature was analyzed. The results showed that the expandin gene TaEXPB8 and TaEXPB10 were cloned from the root of Dongnong Dongmai, a strong cold resistant winter wheat variety, and the two genes encoded 273,271 amino acids, respectively, in order to study the relationship between expandin gene and plant cold resistance. The sequence of TaEXPB8 and TaEXPB10 gene expressed in the root of Dongnongmai 1, a strong cold resistant winter wheat cultivar, could be induced by low temperature stress of 99.2 ~ 99.6C and at -20 鈩，

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