印迹基因CDKN1C对滋养细胞生物学功能的影响

发布时间：2018-02-22 00:39

本文关键词： 印迹基因 CDKNC 滋养细胞　出处：《广东医学》2017年07期 　论文类型：期刊论文

【摘要】：目的研究印迹基因CDKN1C对人滋养细胞生物学功能的影响。方法利用小干扰RNA沉默CDKN1C基因的表达,将人绒毛外滋养细胞(TEV-1细胞株)分为3组,空白组细胞未经转染处理,对照组细胞由不含siRNA的空白脂粒体进行转染,siRNA组细胞由包裹siRNA的脂粒体进行转染。实时荧光定量-PCR(qRTPCR)和免疫印迹(Western blot)检测TEV-1细胞CDKN1C基因的表达水平,CCK-8检测细胞增殖率,流式细胞术检测细胞凋亡及细胞周期,Transwell细胞迁移实验检测细胞侵袭和迁移能力。结果 CDKN1C基因沉默后48h TEV-1细胞早期和晚期凋亡率均降低,细胞增殖率升高;细胞周期G0/G1期比例下降、S期和G2/M期比例增高;细胞迁移和侵袭能力增强(P0.05)。结论印迹基因CDKN1C对人滋养细胞增殖、凋亡、细胞周期、迁移、侵袭等生物学功能有调控作用。
[Abstract]:Objective to study the effect of imprinted gene CDKN1C on the biological function of human trophoblastic cells. Methods Human extracellular trophoblast cell line (TEV-1 cell line) was divided into three groups by silencing the expression of CDKN1C gene by small interfering RNA. The cells in the blank group were not treated with transfection. In the control group, the cells were transfected by lipofectin coated with siRNA. The expression level of CDKN1C gene in TEV-1 cells was detected by real-time fluorescence quantification-PCRnqRTPCRand Western blotting. CCK-8 was used to detect the proliferation rate of TEV-1 cells. Flow cytometry was used to detect cell apoptosis and cell cycle transwell cell migration. Results the early and late apoptosis rate of TEV-1 cells was decreased and the cell proliferation rate was increased 48 hours after CDKN1C gene silencing. The proportion of G _ 0 / G _ 1 phase decreased in S phase and G _ 2 / M phase, and the cell migration and invasion increased (P 0.05). Conclusion the imprinted gene CDKN1C can regulate the biological functions of human trophoblastic cell proliferation, apoptosis, cell cycle, migration and invasion.
【作者单位】：广州医科大学附属第二医院产科;中山大学附属第一医院口腔科;广东省妇幼保健院医学遗传中心;广东省人民医院妇产科;中山大学附属第一医院胎儿医学中心;
【基金】：广东省自然科学基金资助项目(编号:2016A030310176) 广州市科技计划项目(编号:1563000549) 广州医科大学博士启动基金项目(编号:2015C16)
【分类号】：R329.2

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