水稻类病斑突变体wy3的鉴定和基因定位

发布时间：2018-02-22 06:01

本文关键词： 水稻类病斑突变体基因定位　出处：《中国水稻科学》2016年03期 　论文类型：期刊论文

【摘要】：在粳稻品种武育粳3号栽培群体中获得一个类病斑突变体wy3。该突变体类病斑出现于苗期,分蘖期扩散至整张叶片,属于扩散型类病斑突变体。相比野生型,突变体wy3的株高明显降低,有效分蘖数减少,穗长、每穗粒数、结实率均显著降低。遮光处理表明,突变体wy3类病斑的产生受自然光诱导。台盼蓝染色结果表明,类病斑部位有大量的死亡细胞。突变体wy3的光合色素含量和净光合速率较野生型显著降低,SOD、POD、CAT活性和MDA含量均显著高于野生型。遗传分析表明突变体表型受单隐性核基因控制,采用BSA将该基因初步定位在第2染色体短臂端粒附近。采用F2群体中1099株类病斑单株将基因定位在标记W2-17和W2-18之间28kb的物理距离内。测序结果表明,突变体wy3中的LOC_Os02g02000编码区(CDS)第375位碱基C缺失,导致翻译提前终止,突变体中该候选基因为OsHPL3的一个新等位基因。
[Abstract]:A disease-like mutant wy3was obtained from a Japonica rice cultivar Wuyujing 3. The mutant appeared at seedling stage and spread to the whole leaf at tillering stage. The plant height, effective tiller number, panicle length, grain number per panicle and seed setting rate of mutant wy3 were significantly decreased. Shading treatment showed that the occurrence of wy3 disease spot was induced by natural light. The photosynthetic pigment content and net photosynthetic rate of mutant wy3 were significantly lower than those of wild type. The cat activity and MDA content of wy3 were significantly higher than that of wild type. Genetic analysis showed that the mutant surface type was controlled by single recessive gene. The gene was preliminarily located near the short arm telomere on chromosome 2 by BSA. The gene was located within a physical distance of 28kb between W2-17 and W2-18 using 1099 disease-like single plants in F2 population. The deletion of the 375-base C in the LOC_Os02g02000 coding region of the mutant wy3 leads to the early termination of translation. The candidate gene in the mutant is due to a new OsHPL3 allele.
【作者单位】：扬州大学江苏省作物遗传生理重点实验室/教育部植物功能基因组学重点实验室;
【基金】：国家自然科学基金资助项目(31071384,31000533) 江苏省科技支撑计划资助项目(BE2013301) 江苏高校优势学科建设工程资助项目
【分类号】：S511.22

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