牡丹抗逆转录因子基因PsDREB的功能解析

发布时间：2018-02-25 21:12

本文关键词： 牡丹 PsDREB基因酵母单杂交抗逆性　出处：《浙江大学学报(农业与生命科学版)》2016年06期 　论文类型：期刊论文

【摘要】：通过酵母单杂交试验,对牡丹抗逆转录因子基因PsDREB的DNA结合能力及转录激活功能进行分析。结果显示:PsDREB蛋白能够特异性地与顺式作用元件DRE(dehydration responsive element,干旱应答元件)结合,但不能与突变的DRE(mDRE)结合;该蛋白能够激活下游报告基因His的表达,从而使酵母细胞在缺失His的培养基上能够正常生长。说明PsDREB具有转录激活功能。为了进一步研究该转录因子基因PsDREB在植物抗逆反应中的功能,利用农杆菌介导法将其转化到模式植物烟草中,对获得的转基因烟草进行低温、干旱、高盐、脱落酸等胁迫处理。结果发现:与对照相比,超量表达PsDREB基因能够明显提高烟草对逆境胁迫的抗性,尤其是在抗干旱和高盐方面效果显著。
[Abstract]:The ability of DNA binding and transcriptional activation of the stress resistant transcription factor gene (PsDREB) of peony was analyzed by yeast single hybrid test. The results showed that the protein of 1% PsDREB could specifically bind to the cis-acting element DRE(dehydration responsive element (drought response element). The protein activates the expression of downstream reporter gene His. In order to further study the function of the transcription factor gene PsDREB in plant stress resistance, the yeast cells can grow normally on the culture medium without His, which indicates that PsDREB has the function of transcriptional activation. Agrobacterium tumefaciens-mediated transformation of transgenic tobacco into model plant tobacco was carried out under stress of low temperature, drought, high salt, abscisic acid, etc. The results showed that: compared with control, transgenic tobacco was treated with low temperature, drought, high salt, abscisic acid and so on. Overexpression of PsDREB gene could significantly increase the resistance of tobacco to stress, especially in drought and high salinity.
【作者单位】：浙江省农业科学院花卉研究开发中心;
【基金】：浙江省自然科学基金(LQ13C150003)
【分类号】：Q943.2;S685.11

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