奶白菜‘1170’核基因雄性不育系创制与利用

发布时间：2018-02-25 22:12

本文关键词： 奶白菜核基因雄性不育系转育　出处：《沈阳农业大学》2016年硕士论文　论文类型：学位论文

【摘要】：为了改进奶白菜核复等位基因遗传的雄性不育系‘GMS3，园艺学性状,以稳定遗传的奶白菜优异自交系‘1170’为目标品系,采用连续回交定向转育方法选育新的雄性不育系；以转育成的新不育系为亲本,与自交系配制杂交组合,通过品种比较试验,筛选优良杂交种。主要研究结果如下：1.以核不育系‘GMS3’为测交亲本,鉴定待转育可育品系‘1170’的基因型,证明其在核不育位点上为纯合显性的恢复基因型,即MsfMsf。2.根据目标品系的基因型(MsfMsf),设计定向转育方案,将‘GMS3’的雄性不育基因转入目标品系‘1170’中,获得了新的奶白菜核基因雄性不育系,以及其相应的甲型“两用系”和“临时保持系”。3.转育成的新不育系育性鉴定结果表明,其不育株率和不育度均达到了100%,不育性遗传稳定,不育系的植物学性状接近转育目标品系‘1170’，达到了定向转育的效果。4.以转育成的不育系为母本,与10个自交系配制杂交组合,进行品种比较试验,对各杂交组合的农艺性状进行鉴定。综合各杂交组合产量、株形、帮色等调查结果,筛选出2个优异杂交组合‘Z10’和‘Z34’。
[Abstract]:In order to improve the genoallelic male sterile line (GMS3) and horticultural character of Chinese cabbage (Brassica campestris L.), a new male sterile line was selected by continuous backcross directional transformation with the stable inheritance of the excellent inbred line of Chinese cabbage, Qiu 1170'as the target line. The new sterile lines were used as parents to prepare hybrid combinations with inbred lines. The main results were as follows: 1. Using GMS 3'as the test parent, we identified the genotypes of the fertile line, Y1170', which was to be transfered, and the results were as follows: (1) the genetic male sterile line (GMS 3') was used as the test parent to identify the genotypes of the fertile line, Y1170'. It was proved that the GMS gene was homozygous and dominant, that is, MsfMsf.2.According to the genotype of the target strain, MsfMsfGMS3 'gene was transferred into the target line, Y1170', according to the targeted genotype MsfMsfGMS3', and the result showed that the GMS3 gene was homozygous and dominant, I. E. MsfMsf.2. A new genic male sterile line of Chinese cabbage was obtained, and its corresponding A "dual purpose line" and "temporary maintainer line" .3.The results of fertility identification of the new sterile line showed that the sterile plant rate and sterility degree reached 100%, and the genetic stability of sterility was stable. The botanical characters of the male sterile line were close to the target line of transformation, and the effect of directional transfer was achieved. 4. Using the transformed sterile line as the female parent, the hybrid combination was prepared with 10 inbred lines, and the variety comparison experiment was carried out. The agronomic characters of each cross combination were identified. Based on the investigation results of yield, plant shape and band color of each cross combination, two excellent cross combinations, Z10 'and Z34', were screened out.
【学位授予单位】：沈阳农业大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：S634.3

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