异基因造血干细胞移植后NK细胞重建与急性移植物抗宿主病相关性的研究

发布时间：2018-02-26 02:27

  本文关键词： 自然杀伤细胞 急性移植物抗宿主病 异基因造血干细胞移植　出处：《中国实验血液学杂志》2017年02期 　论文类型：期刊论文

【摘要】：目的:探讨异基因造血干细胞移植(allo-HSCT)后NK细胞重建与急性移植物抗宿主病(aGVHD)发生及预后的相关性。方法:采用流式细胞术检测患者移植后30 d外周血中NK细胞的计数及活性,结合临床表现和相关病理指标综合评价患者aGVHD发病情况,分析NK细胞计数和活性与aGVHD发生及预后的相关性。结果:移植后发生aGVHD患者的NK细胞计数和活性与未发生aGVHD患者相比较显著降低,即655±216 cells/μl vs 1169±372 cells/μl(P=0.002)和7.3±3.6%vs 9.0±3.6%(P=0.008);发生Ⅱ-Ⅳ度aGVHD的患者与发生0-Ⅰ度aGVHD的患者相比,NK细胞计数和活性分别为617±220 cells/μl vs 1081±399 cells/μl(P=0.001)和4.2±1.7%vs 8.3±3.5%(P=0.001)。NK细胞计数和活性与aGVHD发病呈中度负相关。生存分析结果显示,Ⅱ-Ⅳ度aGVHD组复发率高(57%vs 5%)(P=0.010),1年无进展生存(PFS)率显著下降(43%vs 84%)(P=0.010)。结论:移植后NK细胞计数和活性与aGVHD的发生及预后存在一定相关性,提示通过监测NK细胞计数和活性可早期识别和预测aGVHD高危患者,也可能为aGVHD的防治提供了新思路。
[Abstract]:Objective: to investigate the relationship between NK cell reconstruction and the occurrence and prognosis of acute graft-versus-host disease (AGV HDD) after allo-HSCT. Methods: the count and activity of NK cells in peripheral blood of patients with allogeneic hematopoietic stem cell transplantation 30 days after transplantation were detected by flow cytometry. To evaluate the incidence of aGVHD combined with clinical manifestations and related pathological indexes. Results: NK cell count and activity in patients with aGVHD after transplantation were significantly lower than those in patients without aGVHD. 655 卤216 cells / 渭 l vs 1169 卤372 cells / 渭 l P0. 002) and 7.3 卤3. 6 vs 9. 0 卤3. 6 P0. 008, NK cell count and activity were 617 卤220 cells / 渭 l vs 1 081 卤399 cells / 渭 l vs 1 081 卤399 cells / 渭 L P0. 001) and 4.2 卤1. 7 vs 8.3 卤3. 5T 0.001. NK cell count and activity were negatively correlated with the incidence of aGVHD. The recurrence rate of 鈪，

本文编号：1536167